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Titolo:
Development of a reverse transcription-nested polymerase chain reaction assay for differential diagnosis of transmissible gastroenteritis virus and porcine respiratory coronavirus from feces and nasal swabs of infected pigs
Autore:
Kim, L; Chang, KO; Sestak, K; Parwani, A; Saif, LJ;
Indirizzi:
Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Dept Vet Prevent Med, Wooster, OH 44691 USA Ohio State Univ Wooster OH USA 44691 t Prevent Med, Wooster, OH 44691 USA Tufts Univ, Sch Vet Med, N Grafton, MA 01536 USA Tufts Univ N Grafton MA USA 01536 v, Sch Vet Med, N Grafton, MA 01536 USA
Titolo Testata:
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
fascicolo: 4, volume: 12, anno: 2000,
pagine: 385 - 388
SICI:
1040-6387(200007)12:4<385:DOARTP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHALLENGE EXPOSURE; SUCKLING PIGS; TGEV; PROTECTION; VIRULENT; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, L Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Dept Vet Prevent Med, Wooster, OH 44691 USA Ohio State Univ Wooster OH USA 44691 nt Med, Wooster, OH 44691 USA
Citazione:
L. Kim et al., "Development of a reverse transcription-nested polymerase chain reaction assay for differential diagnosis of transmissible gastroenteritis virus and porcine respiratory coronavirus from feces and nasal swabs of infected pigs", J VET D INV, 12(4), 2000, pp. 385-388

Abstract

Transmissible gastroenteritis virus (TGEV), a coronavirus, replicates in intestinal enterocytes and causes diarrhea in young pigs. Porcine respiratory coronavirus (PRCV), a spike (S) gene natural deletion mutant of TGEV, hasa respiratory tissue tropism and causes mild or subclinical respiratory infections. Conventional antigen-based diagnostic tests fail to differentiateTGEV and PRCV, and a blocking ELISA test to serologically differentiate TGEV/PRCV-infected pigs is conducted on convalescent serum retrospectively after disease outbreaks. A reverse transcription (RT)-nested polymerase chainreaction (PCR) with primers targeted to the S gene deletion region to differentiate TGEV/PRCV was developed. The specificity of the RT-nested PCR wasconfirmed with reference and recent field strains of TGEV/PRCV, and its sensitivity was analyzed by testing nasal and fecal samples collected from pigs at various days postinoculation (DPI) with TGEV or PRCV. Specific PCR products for TGEV/PRCV were detected only with the homologous reference or field coronaviruses and for 10-14 DPI of pigs with TGEV (feces) or PRCV (nasal samples). The RT-nested PCR assay was more sensitive than antigen-based assays on the basis of duration of virus detection in experimentally infected pigs and was directly applicable to nasal as well as fecal specimens fromthe field.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/01/20 alle ore 20:26:41