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Titolo:
Dissociation of parathyroid hormone and cyclic-3 ', 5 ' amp effects on Na-Pi uptake by cells isolated from proximal straight tubules of rat kidney
Autore:
Rybczynska, A; Angielski, S; Hoppe, A;
Indirizzi:
Med Univ Gdansk, Dept Pathophysiol, PL-80211 Gdansk, Poland Med Univ Gdansk Gdansk Poland PL-80211 ophysiol, PL-80211 Gdansk, Poland Med Univ Gdansk, Dept Clin Biochem, PL-80211 Gdansk, Poland Med Univ Gdansk Gdansk Poland PL-80211 Biochem, PL-80211 Gdansk, Poland Med Univ Gdansk, Med Res Ctr PAN, PL-80211 Gdansk, Poland Med Univ GdanskGdansk Poland PL-80211 Ctr PAN, PL-80211 Gdansk, Poland Med Univ Gdansk, Dept Clin Chem, PL-80211 Gdansk, Poland Med Univ Gdansk Gdansk Poland PL-80211 lin Chem, PL-80211 Gdansk, Poland Med Univ Gdansk, Lab Mol & Cellular Nephrol, PL-80211 Gdansk, Poland Med Univ Gdansk Gdansk Poland PL-80211 Nephrol, PL-80211 Gdansk, Poland
Titolo Testata:
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
fascicolo: 2, volume: 51, anno: 2000,
pagine: 303 - 314
SICI:
0867-5910(200006)51:2<303:DOPHAC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY ALKALOSIS; PHOSPHATE REABSORPTION; OK CELLS; NEPHRON; PTH; PROPRANOLOL; COTRANSPORT; DEPRIVATION; INHIBITION; GLUTAMINE;
Keywords:
rat proximal convoluted and straight tubule cells; sodium-dependent phosphate transport; parathyroid hormone; adenosine 3',5'-cyclic monophosphate; 3-isobutyl-1-methylxanthine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Hoppe, A Med Univ Gdansk, Dept Pathophysiol, PL-80211 Gdansk, Poland Med Univ Gdansk Gdansk Poland PL-80211 PL-80211 Gdansk, Poland
Citazione:
A. Rybczynska et al., "Dissociation of parathyroid hormone and cyclic-3 ', 5 ' amp effects on Na-Pi uptake by cells isolated from proximal straight tubules of rat kidney", J PHYSL PH, 51(2), 2000, pp. 303-314

Abstract

Studies in respiratory alkalotic or short-term phosphate deprived rats raised the possibility that in straight portion of proximal tubules (PST) cAMPmight be not a mediator of PTH in inhibition of phosphate reabsorption. The present experiments directly compared the sensitivity of Na-dependent phosphate [P-32] (Na-Pi) uptake to PTH or cAMP by PCT or PST cells freshly prepared from outer cortex and outer stripe of outer medulla of rat kidney. The purity of the cells was examined by activity of enzymes specific for PST i.e. glutamine synthetase, gamma-glutamyl transpeptidase and creatine kinase, a marker enzyme for medullary thick ascending limb (MTAL) and distal convoluted tubule. Similar inhibition of Na-Pi uptake by 1-34 bPTH by PST and PCT cells was observed: -33.0 and -30.0 % (ns), respectively. In contrast, dibutyryl cAMP decreased Na-Pi uptake only by PCT but not by PST cells: -31.0 and -3.6% (p < 0.02), respectively. The 3-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor, resulted in slight stimulation of Na-Pi uptake by PST but strong inhibition by PCT cells: 7.8 vs -26.0% (p < 0.001). In contrast to PCT in PST cells cAMP seems to play a minor role as a mediator of inhibition of Na-Pi uptake by PTH.

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Documento generato il 04/04/20 alle ore 08:33:14