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Titolo:
Synthesis and functional integration of a neurotransmitter receptor in isolated invertebrate axons
Autore:
Spencer, GE; Syed, NI; van Kesteren, E; Lukowiak, K; Geraerts, WPM; van Minnen, J;
Indirizzi:
Univ Calgary, Hlth Sci Ctr, Neurosci Res Grp, Dept Cell Biol & Anat, Calgary, AB T2N 4N1, Canada Univ Calgary Calgary AB Canada T2N 4N1 Anat, Calgary, AB T2N 4N1, Canada Univ Calgary, Hlth Sci Ctr, Neurosci Res Grp, Dept Physiol & Biophys, Calgary, AB T2N 4N1, Canada Univ Calgary Calgary AB Canada T2N 4N1 ophys, Calgary, AB T2N 4N1, Canada Free Univ Amsterdam, Fac Biol, Dept Mol & Cellular Neurobiol, NL-1081 HV Amsterdam, Netherlands Free Univ Amsterdam Amsterdam Netherlands NL-1081 HV terdam, Netherlands
Titolo Testata:
JOURNAL OF NEUROBIOLOGY
fascicolo: 1, volume: 44, anno: 2000,
pagine: 72 - 81
SICI:
0022-3034(200007)44:1<72:SAFIOA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCAL PROTEIN-SYNTHESIS; LONG-TERM FACILITATION; MESSENGER-RNA; INSITU HYBRIDIZATION; HIPPOCAMPAL-NEURONS; LYMNAEA-STAGNALIS; SYNAPSES; APLYSIA; INDUCTION; DENDRITES;
Keywords:
mRNA; axon; transmitter receptor; protein synthesis; mollusc; electrophysiology; synaptic plasticity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Spencer, GE Univ Calgary, Hlth Sci Ctr, Neurosci Res Grp, Dept Cell Biol &Anat, 3330 Hosp Dr NW, Calgary, AB T2N 4N1, Canada Univ Calgary 3330 Hosp Dr NW Calgary AB Canada T2N 4N1 Canada
Citazione:
G.E. Spencer et al., "Synthesis and functional integration of a neurotransmitter receptor in isolated invertebrate axons", J NEUROBIOL, 44(1), 2000, pp. 72-81

Abstract

Neurotransmitter receptors are considered an important class of membrane proteins that are involved in plasticity-induced changes underlying learningand memory. Recent studies, which demonstrated that the mRNAs encoding forvarious receptor proteins are localized to specific dendritic domains, allude toward the possibility that these membrane bound molecules may be synthesized locally. However, direct evidence for the local axonal or dendritic synthesis and functional integration of receptor proteins in either vertebrates or invertebrates is still lacking. In this study, using an invertebrate model system we provide the first direct evidence that isolated axons (inthe absence of the soma) can intrinsically synthesize and functionally integrate a membrane-bound receptor protein from an axonally injected mRNA. Surgically isolated axons from identified neurons mere injected with mRNA encoding a G-protein-coupled conopressin receptor. Immunocytochemical and electrophysiological techniques were used to demonstrate functional integrationof the receptor protein into the membrane of the isolated axon. Ultrastructural analysis of axonal compartments revealed polyribosomes, suggesting that some components of the protein synthesizing machinery are indeed presentin these extrasomal compartments. Such axonal propensity to locally synthesize and functionally insert transmitter receptors may be instrumental in plasticity induced changes, for instance those that underlie learning and memory. (C) 2000 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:12:45