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Titolo:
Topology of the components of the DNA packaging machinery in the phage phi29 prohead
Autore:
Ibarra, B; Caston, JR; Llorca, O; Valle, M; Valpuesta, JM; Carrascosa, JL;
Indirizzi:
CSIC, Dept Macromol Struct, Ctr Nacl Biotecnol, Madrid 28049, Spain CSIC Madrid Spain 28049 Struct, Ctr Nacl Biotecnol, Madrid 28049, Spain
Titolo Testata:
JOURNAL OF MOLECULAR BIOLOGY
fascicolo: 5, volume: 298, anno: 2000,
pagine: 807 - 815
SICI:
0022-2836(20000519)298:5<807:TOTCOT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAD-TAIL CONNECTOR; BACTERIOPHAGE-PHI-29 CONNECTOR; 3D RECONSTRUCTION; PORTAL PROTEIN; RNA; RESOLUTION; VIRUS; REPLICATION; BINDING; REVEALS;
Keywords:
electron microscopy; image processing; phage phi 29; viral proheads; antibody labeling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Carrascosa, JL CSIC, Dept Macromol Struct, Ctr Nacl Biotecnol, Campus UnivAutonoma Madrid, Madrid 28049, Spain CSIC Campus Univ Autonoma Madrid Madrid Spain 28049 Spain
Citazione:
B. Ibarra et al., "Topology of the components of the DNA packaging machinery in the phage phi29 prohead", J MOL BIOL, 298(5), 2000, pp. 807-815

Abstract

Chromosome condensation inside dsDNA viral particles is a complex process requiring the coordinated action of several viral components. The similarity of the process in different viral systems has led to the suggestion that there is a common underlying mechanism for DNA packaging, in which the portal vertex or connector plays a key role. We have studied the topology of the packaging machinery using a number of antibodies directed against different domains of the connector. The charged aminoterminal, the carboxyl-terminal, and the RNA binding domain are accessible areas in the connector assembled into the prohead, while the domains corresponding to the 12 large appendages of the connector are buried inside the prohead. Furthermore, while the antibodies against the carboxyl and amino-terminal do not affect the packaging reaction, incubation of proheads with antibodies against the RNA binding domain abolishes the packaging activity. The comparison of the three-dimensional reconstructions of bacteriophage phi 29 proheads with proheads devoid of their specific pRNA by RNase treatment shows that this treatment removes structural elements of the distal vertex of the portal structure, suggesting that the pRNA required for packaging is located at the open gate ofthe channel in the narrow side of the connector. (C) 2000 Academic Press.

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Documento generato il 11/07/20 alle ore 15:30:38