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Titolo:
Slowly inactivating component of sodium current in ventricular myocytes isdecreased by diabetes and partially inhibited by known Na+-H+ exchange blockers
Autore:
Chattou, S; Coulombe, A; Diacono, J; Le Grand, B; John, G; Feuvray, D;
Indirizzi:
Univ Paris 11, Lab Physiol Cellulaire, UFR Orsay, F-91405 Orsay, France Univ Paris 11 Orsay France F-91405 ire, UFR Orsay, F-91405 Orsay, France Univ Paris 11, Hop Marie Lannelongue, CNRS ESA 8078, F-91405 Orsay, FranceUniv Paris 11 Orsay France F-91405 CNRS ESA 8078, F-91405 Orsay, France Ctr Rech Pierre Fabre, Div Maladies Cardiovasc 2, F-81106 Castres, France Ctr Rech Pierre Fabre Castres France F-81106 2, F-81106 Castres, France
Titolo Testata:
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
fascicolo: 7, volume: 32, anno: 2000,
pagine: 1181 - 1192
SICI:
0022-2828(200007)32:7<1181:SICOSC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT HEARTS; INTRACELLULAR NA+; CHANNEL BLOCKADE; SKELETAL-MUSCLE; REPERFUSION; ISCHEMIA; LYSOPHOSPHATIDYLCHOLINE; INJURY; PH; CARDIOMYOCYTES;
Keywords:
cardiac myocytes; EIPA; HOE 642; HOE 694; slowly inactivating component of sodium current; I-NaL; streptozotocin-induccd diabetes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Feuvray, D Univ Paris 11, Lab Physiol Cellulaire, UFR Orsay, Bat 443, F-91405 Orsay, France Univ Paris 11 Bat 443 Orsay France F-91405 91405 Orsay, France
Citazione:
S. Chattou et al., "Slowly inactivating component of sodium current in ventricular myocytes isdecreased by diabetes and partially inhibited by known Na+-H+ exchange blockers", J MOL CEL C, 32(7), 2000, pp. 1181-1192

Abstract

Recent evidence has suggested a major role for a slowly inactivating component of Na+ current (I-NaL) as a contributor to ischemic Na+ loading. The purposes of this study were to investigate veratrine and lysophosphatidylcholine (LPC)-induced I-NaL in single ventricular myocytes of normal and diabetic rats and Co analyse the effects on this current of three pharmacological agents, known as Na+/H+ exchange inhibitors, whose selectivity has been questioned in several studies, A decrease in Na+/H+ exchange activity has been previously shown to be associated with diabetes, and this has been foundto confer some protection to the diabetic heart after an episode of ischemia/reperfusion. Recordings were made using the whole-cell patch-clamp technique. I-NaL was stimulated either by veratrine (100 mg/ml) or by LPC (10 mumol/l) applied extracellularly. Veratrine as well as LPC-induced I-NaL wasfound to be significantly decreased in ventricular myocytes isolated from diabetic rat hearts. Veratrine- and LPC-induced I-NaL in ventricular myocytes of normal rats was significantly (in the range 10(-7) to 10(-4) mol/l) inhibited by the Na+/H+ exchange blockers HOE 694, EIPA and HOE 642. HOE 694was the most potent inhibitor, followed by the amiloride derivative EIPA and HOE 642. The sensitivity of veratrine-induced I-NaL to inhibition by HOE694 and EIPA was markedly reduced in diabetic ventricular myocytes, with no observed inhibition by HOE 642. These data may have important implications as to the protection that may be afforded against ischemic and reperfusion injury, especially during ischemia and when ischemia occurs in a diabeticsituation. (C) 2000 Academic Press.

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Documento generato il 28/03/20 alle ore 23:38:36