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Titolo:
Detection of reverse transcriptase activity in the serum of patients with motor neurone disease
Autore:
Andrews, WD; Tuke, PW; Al-Chalabi, A; Gaudin, P; Ijaz, S; Parton, MJ; Garson, JA;
Indirizzi:
Royal Free & Univ Coll Med Sch, Dept Virol, London W1P 6DB, England Royal Free & Univ Coll Med Sch London England W1P 6DB n W1P 6DB, England Inst Psychiat, Dept Clin Neurosci, London, England Inst Psychiat London England chiat, Dept Clin Neurosci, London, England
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 4, volume: 61, anno: 2000,
pagine: 527 - 532
SICI:
0146-6615(200008)61:4<527:DORTAI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYOTROPHIC-LATERAL-SCLEROSIS; TAX-REX DNA; LEUKEMIA-VIRUS; ASSAY; HTLV; ANTIBODIES; RNA; PCR; POLYMERASE; INFECTION;
Keywords:
amyotrophic lateral sclerosis; motor neurone disease; product-enhanced reverse transcriptase assay; retrovirus; reverse transcriptase; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Garson, JA Royal Free & Univ Coll Med Sch, Dept Virol, Windeyer Bldg,46 Cleveland St,London W1P 6DB, England Royal Free & Univ Coll Med Sch Windeyer Bldg,46 Cleveland St London England W1P 6DB
Citazione:
W.D. Andrews et al., "Detection of reverse transcriptase activity in the serum of patients with motor neurone disease", J MED VIROL, 61(4), 2000, pp. 527-532

Abstract

The recognition that both human and murine retroviruses can cause motor neurone disease-like syndromes has raised the possibility that a retrovirus may be involved in the aetiology of motor neurone disease. This possibility was explored by looking for evidence of reverse transcriptase in the serum of motor neurone disease patients. Sera from 56 patients with motor neuronedisease and 58 controls were tested by the product-enhanced reverse transcriptase assay, a technique that is approximately a million fold more sensitive than conventional reverse transcriptase assays and capable of detectingvery low numbers of retroviral particles. Cell-free reverse transcriptase activity was detected in the serum of 33 of the 56 motor neurone disease patients (59%) but in only 3 of the controls (P < 0.00001). The reverse transcriptase activity was detectable in the presence of a large excess of an effective inhibitor of human cellular DNA polymerases and was therefore tentatively considered to be compatible with a retroviral origin. The reverse transcriptase activity, however, was not found to be due to the presence of known human exogenous retroviruses including HIV-1, HIV-2, HTLV-I, HTLV-II, HRV-5 or human foamy virus, as assessed by PCR-based assays. Further investigations will be required to determine the source of the reverse transcriptase activity observed in these motor neurone disease patient sera. J. Med. Virol. 61:527-532, 2000. (C) 2000 Wiley-Liss.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:30:45