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Titolo:
Effects of selective protein kinase C inhibitors on the proteolytic down-regulation of L-selectin from chemoattractant-activated neutrophils
Autore:
Alexander, SR; Kishimoto, TK; Walcheck, B;
Indirizzi:
Univ Minnesota, Ctr Immunol, St Paul, MN USA Univ Minnesota St Paul MN USA iv Minnesota, Ctr Immunol, St Paul, MN USA Boehringer Ingelheim Pharmaceut Inc, Ridgefield, CT 06877 USA Boehringer Ingelheim Pharmaceut Inc Ridgefield CT USA 06877 CT 06877 USA
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 3, volume: 67, anno: 2000,
pagine: 415 - 422
SICI:
0741-5400(200003)67:3<415:EOSPKC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
METHIONYL-LEUCYL-PHENYLALANINE; MEMBRANE-PROXIMAL CLEAVAGE; NODE HOMING RECEPTOR; ADHESION MOLECULE; LEUKOCYTE ADHESION; ENDOTHELIAL-CELLS; CALPHOSTIN-C; DEPENDENT MECHANISM; CHEMOTACTIC FACTORS; PHOSPHOLIPASE-D;
Keywords:
formyl peptide; phorbol 12-myristate 13-acetate; Mac-1; shedding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Walcheck, B Univ Minnesota, Dept Vet Pathobiol, 1988 Fitch Ave, St Paul, MN 55108 USA Univ Minnesota 1988 Fitch Ave St Paul MN USA 55108 N 55108 USA
Citazione:
S.R. Alexander et al., "Effects of selective protein kinase C inhibitors on the proteolytic down-regulation of L-selectin from chemoattractant-activated neutrophils", J LEUK BIOL, 67(3), 2000, pp. 415-422

Abstract

The signaling factors that direct the rapid shedding of L-selectin from neutrophils upon chemoattractant stimulation are poorly understood. Protein kinase C (PKC) has been implicated, yet previous studies have relied on the use of phorbol esters and nonselective kinase inhibitors, We treated neutrophils with various selective kinase inhibitors to evaluate their effects onN-formylmethionyl-leucyl-phenylalanine (fMLP)-induced L-selectin shedding. We found that three selective inhibitors of PKC, structurally related to staurosporine, largely blocked both fMLP- and phorbol 12-myristate 13-acetate (PMA)-induced L-selectin shedding; however; these inhibitors did not affect fMLP-induced up-regulation of Mac-1 (CD11b/CD18) expression, which has been shown not to involve PKC. Other selective serine, threonine, and tyrosine kinase inhibitors were found not to block fMLP-induced L-selectin shedding. These findings provide more definitive evidence for the role of PKC in chemoattractant-induced L-selectin proteolysis. It is interesting that certain highly selective PKC inhibitors, not structurally related to staurosporine, were found to directly induce L-selectin shedding from neutrophils.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 03:52:50