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Titolo:
Accelerated telomere shortening and senescence in human pancreatic islet cells stimulated to divide in vitro
Autore:
Halvorsen, TL; Beattie, GM; Lopez, AD; Hayek, A; Levine, F;
Indirizzi:
Univ Calif San Diego, Ctr Mol Genet, La Jolla, CA 92093 USA Univ Calif SanDiego La Jolla CA USA 92093 Genet, La Jolla, CA 92093 USA Univ Calif San Diego, Whittier Inst Diab, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 t Diab, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF ENDOCRINOLOGY
fascicolo: 1, volume: 166, anno: 2000,
pagine: 103 - 109
SICI:
0022-0795(200007)166:1<103:ATSASI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LENS EPITHELIAL-CELLS; KINASE INHIBITOR P16; HUMAN FETAL PANCREAS; HUMAN FIBROBLASTS; LIFE-SPAN; DONOR AGE; REPLICATIVE SENESCENCE; ENDOCRINE-CELLS; CULTURE; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Levine, F Univ Calif San Diego, Ctr Mol Genet, Room 122, La Jolla, CA 92093 USA Univ Calif San Diego Room 122 La Jolla CA USA 92093 CA 92093 USA
Citazione:
T.L. Halvorsen et al., "Accelerated telomere shortening and senescence in human pancreatic islet cells stimulated to divide in vitro", J ENDOCR, 166(1), 2000, pp. 103-109

Abstract

Widespread application of beta-cell replacement strategies for diabetes isdependent upon the availability of an unlimited supply of cells exhibitingappropriate glucose-responsive insulin secretion. Therefore, a great deal of effort has been focused on understanding the factors that control p-cen growth. Previously, we found that human beta-cell-enriched islet cultures can be stimulated to proliferate, but expansion was limited by growth arrestafter 10-15 cell divisions. Here, we have investigated the mechanism behind the growth arrest. Our studies, including analyses of the expression of senescence-associated beta-galactosidase, p16(INK4a) levels, and telomere lengths, indicate that cellular senescence is responsible for limiting the number of cell divisions that human beta-cells can undergo. The senescent phenotype was not prevented by retroviral transduction of the hTERT gene, although telomerase activity was induced. These results have implications for the use of primary human islet cells in cell transplantation therapies for diabetes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:29:57