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Titolo:
Applications of a sulfonated-polymer wall-modified open-tubular fused-silica capillary in capillary zone electrophoretic separations
Autore:
Minnoor, E; Liu, Y; Pietrzyk, DJ;
Indirizzi:
Univ Iowa, Dept Chem, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 Iowa, Dept Chem, Iowa City, IA 52242 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 884, anno: 2000,
pagine: 297 - 309
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROOSMOTIC FLOW; COATED CAPILLARY; ZWITTERIONIC SURFACTANTS; MIGRATION BEHAVIOR; INORGANIC ANIONS; ACIDIC SOLUTION; BASIC-PROTEINS; BUFFER CATION; PH HYSTERESIS; SELECTIVITY;
Keywords:
capillary columns; buffer composition; sulfonic acid bonded phase capillaries; inorganic anions; organic acids; organic bases; alkylsulfonic acids; benzenesulfonic acids; sulfa drugs; pyridines; anilines; peptides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Pietrzyk, DJ Univ Iowa, Dept Chem, Iowa City, IA 52242 USA Univ Iowa IowaCity IA USA 52242 em, Iowa City, IA 52242 USA
Citazione:
E. Minnoor et al., "Applications of a sulfonated-polymer wall-modified open-tubular fused-silica capillary in capillary zone electrophoretic separations", J CHROMAT A, 884(1-2), 2000, pp. 297-309

Abstract

A fused-silica capillary that is wall-modified via chemically bonding a sulfonated polymer to the capillary wall has a uniform negative charge density on its surface and produces an electroosmotic flow (EOF) greater than 4(.)10(-4) cm(2) V-1 s(-1) The EOF is nearly independent of buffer pH over thepH range of 2 to 10 and is lower than the EOF obtained for the bare fused-silica capillary at the more basic pH but is higher at the more acidic buffer pH. Optimization of buffer pH can be based on analyte pK(a) values to improve the overall quality of the capillary zone electrophoresis (CZE) separation of complex mixtures of weak acid and base analytes. Because of the high EOF in an acidic buffer, the capillary is useful for the separation of weak organic bases which are in their cation forms in the acidic buffer. EOFfor the sulfonic acid bonded phase capillary can be adjusted via buffer additives such as organic solvent, tetraalkylammonium salts, multivalent cations and alkylsulfonic acids. The advantages of utilizing buffer pH and the EOF buffer modifiers to enhance migration time, selectivity, and resolutionin CZE separations with this capillary are illustrated using a series of test analyte mixtures of inorganic anions, carboxylic acids, alkylsulfonic acids, benzenesulfonic acids, sulfas, pyridines, anilines or small-chain peptides. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:07:02