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Titolo:
Elution mechanism in electrostatic ion chromatography with histidine as anisoelectric ampholytic mobile phase
Autore:
Macka, M; Haddad, PR;
Indirizzi:
Univ Tasmania, Sch Chem, Hobart, Tas 7001, Australia Univ Tasmania HobartTas Australia 7001 Chem, Hobart, Tas 7001, Australia
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 884, anno: 2000,
pagine: 287 - 296
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAPILLARY-ZONE-ELECTROPHORESIS; CONDUCTIVITY DETECTION; BUFFERS; SEPARATION; OLIGONUCLEOTIDES; ANIONS; DNA; PEPTIDES; CATIONS; ELUENTS;
Keywords:
electrostatic ion chromatography; stationary phases; LC; ampholytes; mobile phase composition; inorganic anions; inorganic cations;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Haddad, PR Univ Tasmania, Sch Chem, GPO Box 252-75, Hobart, Tas 7001, Australia Univ Tasmania GPO Box 252-75 Hobart Tas Australia 7001 ustralia
Citazione:
M. Macka e P.R. Haddad, "Elution mechanism in electrostatic ion chromatography with histidine as anisoelectric ampholytic mobile phase", J CHROMAT A, 884(1-2), 2000, pp. 287-296

Abstract

Electrostatic ion chromatography (EIC) using a zwitterionic stationary phase (formed by coating a C-18 material with a hydrophobic zwitterionic surfactant) was studied with a mobile phase comprising an aqueous solution of histidine at the pH of its isoelectric point, together with non-suppressed conductometric detection. Anions and cations were found to be eluted as separate peaks, unlike the elution behaviour observed on the same system when pure water was used as mobile phase. An explanation was suggested in terms ofprotonation equilibria of the overall uncharged histidine to form small amounts of histidine cations and anions in the mobile phase which could act as counterions for analyte anions and cations. This suggestion was supportedby measured pH changes occurring in the bands of eluted analyte anions (a decreased pH compared to the mobile phase) and cations tan increased pH compared to the mobile phase). The analytical potential of this type of EIC isdiscussed. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 19:57:39