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Titolo:
The gene encoding the mitogen-responsive phosphoprotein Dab2 is differentially regulated by GATA-6 and GATA-4 in the visceral endoderm
Autore:
Morrisey, EE; Musco, S; Chen, MYZ; Lu, MM; Leiden, JM; Parmacek, MS;
Indirizzi:
Univ Penn, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PAUSA 19104 Dept Med, Philadelphia, PA 19104 USA Harvard Univ, Sch Publ Hlth, Dept Med, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 ubl Hlth, Dept Med, Boston, MA 02115 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 26, volume: 275, anno: 2000,
pagine: 19949 - 19954
SICI:
0021-9258(20000630)275:26<19949:TGETMP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR GATA-1; ZINC-FINGER PROTEIN; HEART TUBE FORMATION; CAENORHABDITIS-ELEGANS; VENTRAL MORPHOGENESIS; CELLS; FAMILY; HEMATOPOIESIS; EXPRESSION; DROSOPHILA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Parmacek, MS Univ Penn, Dept Med, 9123 Founders Pavil,3400 Spruce St, Philadelphia, PA 19104 USA Univ Penn 9123 Founders Pavil,3400 Spruce St Philadelphia PA USA 19104
Citazione:
E.E. Morrisey et al., "The gene encoding the mitogen-responsive phosphoprotein Dab2 is differentially regulated by GATA-6 and GATA-4 in the visceral endoderm", J BIOL CHEM, 275(26), 2000, pp. 19949-19954

Abstract

Gene targeting studies have demonstrated that the zinc finger transcription factor GATA-6 lies upstream in a transcriptional cascade that controls differentiation of the visceral endoderm, To understand the function of GATA-6 in the visceral endoderm and to identify genes regulated by GATA-6 in this tissue, subtractive hybridization was performed using template cDNAs derived from differentiated wild-type embryonic stem (ES) cells and GATA-6(-/-)ES cells, respectively. These analyses revealed that the gene encoding Dab2, a mitogen-responsive phosphoprotein, is differentially expressed in wild-type and GATA-6-deficient ES cells. Consistent with these findings, Dab2 is expressed in the visceral endoderm of wild-type embryos but not in the visceral endoderm of GATA-6-deficient embryos. Cotransfection experiments demonstrate that the human Dab2 promoter can be transactivated by forced expression of GATA-6 in NIH-3T3 cells. In contrast, forced expression of GATA-4 does not transactivate the human Dab2 promoter and Dab2 is expressed in thevisceral endoderm of GATA-4 null embryos. Surprisingly, the specificity ofGATA-6-induced transactivation of the Dab2 promoter is not mediated through its zinc finger DNA-binding domain. Taken together, these data demonstrate that the mitogen-responsive phosphoprotein Dab2 is a downstream target ofGATA-6 in the visceral endoderm, Moreover, these data demonstrate that molecular mechanisms have evolved that direct, and distinguish, the functionalspecificity of GATA family members when they are developmentally coexpressed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 13:42:02