Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The alpha 3 beta 1 integrin is associated with mammary carcinoma cell metastasis, invasion, and gelatinase B (MMP-9) activity
Autore:
Morini, M; Mottolese, M; Ferrari, N; Ghiorzo, F; Buglioni, S; Mortarini, R; Noonan, DM; Natali, PG; Albini, A;
Indirizzi:
Ist Nazl Ric Canc, Ctr Biotecnol Avanzate, I-16132 Genoa, Italy Ist Nazl Ric Canc Genoa Italy I-16132 nol Avanzate, I-16132 Genoa, Italy Ist Nazl Ric Canc, Mol Biol Lab, I-16132 Genoa, Italy Ist Nazl Ric Canc Genoa Italy I-16132 Mol Biol Lab, I-16132 Genoa, Italy Ist Nazl Tumori, I-20133 Milan, Italy Ist Nazl Tumori Milan Italy I-20133 st Nazl Tumori, I-20133 Milan, Italy
Titolo Testata:
INTERNATIONAL JOURNAL OF CANCER
fascicolo: 3, volume: 87, anno: 2000,
pagine: 336 - 342
SICI:
0020-7136(20000801)87:3<336:TA3B1I>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR PROGRESSION; BREAST-CANCER; ADHESION MOLECULES; MATRIX METALLOPROTEINASES; GLIOMA-CELLS; EXPRESSION; INHIBITION; MIGRATION; HETERODIMER; ANTI-ALPHA-3;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Albini, A Ist Nazl Ric Canc, Ctr Biotecnol Avanzate, Largo Rosanna Benzi 10, I-16132Genoa, Italy Ist Nazl Ric Canc Largo Rosanna Benzi 10 Genoa Italy I-16132 ly
Citazione:
M. Morini et al., "The alpha 3 beta 1 integrin is associated with mammary carcinoma cell metastasis, invasion, and gelatinase B (MMP-9) activity", INT J CANC, 87(3), 2000, pp. 336-342

Abstract

The alpha 3 beta 1 integrin is elevated in several types of metastatic tumor and has been associated with increased migration and invasion. Our analysis of a series of mammary carcinomas of different histotypes and their corresponding metastases demonstrated significantly increased expression of alpha 3 beta 1 I in the tumor metastases. We therefore studied alpha 3 beta 1expression of several human breast carcinoma cell lines and its association with the invasive phenotype. The MDA-MB-231 cell line expressed high levels of the beta 1, alpha 2, alpha 3, alpha 5, and alpha 6 integrin subunits along with moderate levels of the alpha v beta 3 integrin, This line was highly migratory and the most invasive using a chemo-invasion assay. In contrast, the other lines tested, MDA-MB-145, MCF-7, and SK-BR-3, showed lower migratory and invasive activity and reduced alpha 3 integrin subunit expression. Metalloproteases capable of degrading collagen IV are necessary for the invasive process. RT-PCR showed that MDA-MB-231 cells expressed MMP-9, but not MMP-2, gelatinase/collagenase IV. Gelatin zymography demonstrated that invading MDA-MB-231 cells released high levels of MMP-9 gelatinase activity. A direct role for this gelatinase in MDA-MB-231 cell invasion was confirmed by inhibition of invasion using the metalloprotease inhibitor Batimastat. Treatment of MDA-MB-231 cells with a function-blocking anti-alpha 3 antibody strongly inhibited migration and invasion. This correlated with a marked reduction in MMP-9 activity produced by MDA-MB-231 cells, suggesting a role for alpha 3 beta 1 ligand binding in cell signaling and regulation of extracellular matrix degradation. Int. J. Cancer 87:336-342, 2000. (C) 2000Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 07:30:49