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Titolo:
Antineutrophil antibodies associated with ulcerative colitis interact withthe antigen(s) during the process of apoptosis
Autore:
Mallolas, J; Esteve, M; Rius, E; Cabre, E; Gassull, MA;
Indirizzi:
Hosp Univ Germans Trias & Pujol, Dept Gastroenterol, Badalona 08916, Catalonia, Spain Hosp Univ Germans Trias & Pujol Badalona Catalonia Spain 08916nia, Spain Univ Barcelona, Sch Med, Inst Invest Biomed August Pi & Sunyer, Dept Cellular Biol & Pathol, E-08036 Barcelona, Spain Univ Barcelona Barcelona Spain E-08036 Pathol, E-08036 Barcelona, Spain
Titolo Testata:
GUT
fascicolo: 1, volume: 47, anno: 2000,
pagine: 74 - 78
SICI:
0017-5749(200007)47:1<74:AAAWUC>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY BOWEL-DISEASE; PROGRAMMED CELL-DEATH; SYSTEMIC LUPUS-ERYTHEMATOSUS; CYTOPLASMIC ANTIBODIES; NEUTROPHILS; DNA; AUTOANTIBODIES; NEPHRITIS; PROTEINS; ALPHA;
Keywords:
antineutrophil cytoplasmic antibody; antigen; ulcerative colitis; apoptosis; humoral immunity; immunofluorescent laser confocal microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Gassull, MA Hosp Univ Germans Trias & Pujol, Dept Gastroenterol, CarreteraCanyet S-N,Badalona 08916, Catalonia, Spain Hosp Univ Germans Trias & Pujol Carretera Canyet S-N Badalona Catalonia Spain 08916
Citazione:
J. Mallolas et al., "Antineutrophil antibodies associated with ulcerative colitis interact withthe antigen(s) during the process of apoptosis", GUT, 47(1), 2000, pp. 74-78

Abstract

Background-Cell death bg: apoptosis seems to be an important mechanism fortranslocation to the cell surface of a variety of intracellular componentscapable of inducing autoantibody production. Aims-To identify the cellularlocation of antigen (Ag)-antineutrophil cytoplasmic antibodies (ANCA) in non-apoptotic human neutrophils, and to assess if ANCA associated with ulcerative colitis reacts with neutrophil antigen(s) during neutrophil apoptosis. The cellular distribution of Ag-ANCA in apoptotic neutrophils was also investigated. Methods-Sera from 18 ulcerative colitis patients known to be positive for perinuclear IgG-ANCA (titre greater than or equal to 1/320), as assessed byindirect immunofluorescence (IIF), were analysed by immunofluorescent confocal laser scanning microscopy. ANCA were identified with fluorescein isothiocyanate (FITC) and tetramethylrhodamine isothiocyanate (TRITC) in non-apoptotic and apoptotic neutrophils, respectively. Apoptotic and non-apoptoticDNA was labelled with FITC and propidium iodide, respectively. Cycloheximide was added to polymorphonuclear leucocyte culture to induce apoptosis. Results-Three patterns of scanning laser immunofluorescence microscopy in non-apoptotic neutrophils were observed with respect to cellular ulcerativecolitis associated ANCA distribution: (1) diffuse nuclear localisation (16.7%); (2) nuclear localisation in the nuclear periphery (50%); and (3) mixed nuclear and cytoplasmic localisation (33.4%). In all sera ANCA fluorescence colocalised almost completely with apoptotic DNA, with persistence of a diffuse and intense fluorescence. No significant changes in ANCA titres were found in non-apoptotic neutrophils. Conclusions-The antigen(s) of ANCA associated with ulcerative colitis seems to be localised in most cases in the neutrophil nucleus. The almost identical colocalisation of ANCA and apoptotic cleaved DNA suggests that intracellular DNA redistribution during neutrophil apoptosis may play a role in antigen exposure to the immune system and ANCA production in ulcerative colitis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:43:33