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Titolo:
3-DIMENSIONAL GROWTH-PATTERNS OF VARIOUS HUMAN TUMOR-CELL LINES IN SIMULATED MICROGRAVITY OF A NASA BIOREACTOR
Autore:
INGRAM M; TECHY GB; SAROUFEEM R; YAZAN O; NARAYAN KS; GOODWIN TJ; SPAULDING GF;
Indirizzi:
HUNTINGTON MED RES INST,99 N EL MOLINO AVE PASADENA CA 91101 CLEAR LAKE MED FDN HOUSTON TX 00000 NASA,LYNDON B JOHNSON SPACE CTR HOUSTON TX 77058
Titolo Testata:
In vitro cellular & developmental biology. Animal
fascicolo: 6, volume: 33, anno: 1997,
pagine: 459 - 466
SICI:
1071-2690(1997)33:6<459:3GOVHT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROTATING-WALL VESSEL; MULTICELLULAR SPHEROIDS; TISSUE-CULTURE; CANCER; CD44; DIFFERENTIATION; CARCINOMA; ADHESION; MODEL;
Keywords:
SPHEROID ARCHITECTURE; SUSPENSION CULTURE; LOW-SHEAR CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
M. Ingram et al., "3-DIMENSIONAL GROWTH-PATTERNS OF VARIOUS HUMAN TUMOR-CELL LINES IN SIMULATED MICROGRAVITY OF A NASA BIOREACTOR", In vitro cellular & developmental biology. Animal, 33(6), 1997, pp. 459-466

Abstract

Growth patterns of a number of human tumor cell lines that form three-dimensional structures of various architectures when cultured withoutcarrier beads in a NASA rotary cell culture system are described and illustrated. The culture system, which was designed to mimic microgravity, maintained cells in suspension under very low-shear stress throughout culture. Spheroid (particulate) production occurred within a few hours after culture was started, and spheroids increased in size by cell division and fusion of small spheroids, usually stabilizing at a spheroid diameter of about 0.5 mm. Architecture of spheroids varied withcell type. Cellular interactions that occurred in spheroids resulted in conformation and shape changes of cells, and some cell lines produced complex, epithelial-like architectures. Expression of the cell adhesion molecules, CD44 and E cadherin, was upregulated in the three-dimensional constructs. Coculture of fibroblast spheroids with PC3 prostate cancer cells induced tenascin expression by the fibroblasts underlying the adherent prostate epithelial cells. Invasion of the fibroblast spheroids by the malignant epithelium was also demonstrated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 21:54:53