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Titolo:
Orientation specificity of the Lactococcus lactis Chi site
Autore:
El Karoui, M; Schaeffer, M; Biaudet, V; Bolotin, A; Sorokin, A; Gruss, A;
Indirizzi:
INRA, Lab Genet Appl, URLGA, F-78352 Jouy En Josas, France INRA Jouy En Josas France F-78352 , URLGA, F-78352 Jouy En Josas, France INRA, Lab Genet Microbienne, F-78352 Jouy En Josas, France INRA Jouy En Josas France F-78352 obienne, F-78352 Jouy En Josas, France
Titolo Testata:
GENES TO CELLS
fascicolo: 6, volume: 5, anno: 2000,
pagine: 453 - 461
SICI:
1356-9597(200006)5:6<453:OSOTLL>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINATION HOTSPOT-CHI; ESCHERICHIA-COLI GENOME; GRAM-POSITIVE BACTERIA; SEQUENCE PROTECTS DNA; MUTANT RECBCD ENZYME; BACILLUS-SUBTILIS; EXONUCLEOLYTIC DEGRADATION; HAEMOPHILUS-INFLUENZAE; RECB(2109)CD ENZYME; REPLICATION FORKS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Gruss, A INRA, Lab Genet Appl, URLGA, Domaine Vilvert, F-78352 Jouy En Josas, France INRA Domaine Vilvert Jouy En Josas France F-78352 Josas, France
Citazione:
M. El Karoui et al., "Orientation specificity of the Lactococcus lactis Chi site", GENES CELLS, 5(6), 2000, pp. 453-461

Abstract

Background: In Escherichia coli, the Chi sequence modulates the activity of RecBCD, a powerful double-stranded (ds) DNA exonuclease/helicase. Chi attenuates RecBCD exonuclease activity and stimulates homologous recombinationin an orientation-dependent manner. Chi(Ec) is frequent and over-represented on its genome, which is thought to be related to its role in dsDNA breakrepair. We previously identified a Chi-like sequence (referred to as Chi(Ll)) and an exonuclease/helicase in the Gram-positive bacterium Lactococcus lactis. Chi(Ll) and RexAB are functional analogues of Chi(Ec) and RecBCD. Results: We report that Chi(Ll) attenuates RexAB exonuclease activity and stimulates homologous recombination in an orientation-dependent manner. Analysis of Chi(Ll) distribution on the L. lactis chromosome reveals that Chi(Ll) is frequent, highly over-represented, and oriented with respect to the direction of replication. Conclusions: Our results show that a single orientation of Chi(Ll) interacts with RexAB. The active orientation is preferentially found on the replication leading strand of the L. lactis genome, consistent with a primary role of Chi(Ll) in repair of dsDNA breaks at the replication fork. We propose that orientation-dependence of Chi activity and over-representation of Chi sequences on bacterial genomes may be conserved properties of exonuclease/helicase-Chi couples. Other properties of the Chi sequence distribution on the genomes might reflect more specific characteristics of each couple and of the host.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:22:18