Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Relation between colonic inflammation severity and total low-molecular-weight antioxidant profiles in experimental colitis
Autore:
Blau, S; Kohen, R; Bass, P; Rubinstein, A;
Indirizzi:
Hebrew Univ Jerusalem, Sch Pharm, Fac Med, IL-91120 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91120 -91120 Jerusalem, Israel Univ Wisconsin, Sch Pharm, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Sch Pharm, Madison, WI 53706 USA
Titolo Testata:
DIGESTIVE DISEASES AND SCIENCES
fascicolo: 6, volume: 45, anno: 2000,
pagine: 1180 - 1187
SICI:
0163-2116(200006)45:6<1180:RBCISA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRINITROBENZENE SULFONIC-ACID; REACTIVE OXYGEN METABOLITES; INDUCED OXIDATIVE STRESS; HAPTEN-INDUCED MODEL; EPITHELIAL L2 CELLS; CLOSED-HEAD INJURY; BOWEL-DISEASE; CYCLIC VOLTAMMETRY; GLUTATHIONE SYNTHESIS; FREE-RADICALS;
Keywords:
experimental colitis; dinitrobenzene sulfonic-acid; low-molecular-weight antioxidants; glutathione; oxidative stress; cyclic voltammetry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Rubinstein, A Hebrew Univ Jerusalem, Sch Pharm, Fac Med, POB 12065, IL-91120 Jerusalem, Israel Hebrew Univ Jerusalem POB 12065 Jerusalem Israel IL-91120 l
Citazione:
S. Blau et al., "Relation between colonic inflammation severity and total low-molecular-weight antioxidant profiles in experimental colitis", DIG DIS SCI, 45(6), 2000, pp. 1180-1187

Abstract

Tissue antioxidant status is altered as a response to oxidative stress. This oxidative stress, caused by reactive oxygen species, is associated with inflammatory bowel disease (IBD). Our aim was to examine the relationship between total tissue low-molecular-weight antioxidant (LMWA) profile and inflammation severity in dinitrobenzene sulfonic acid (DNBS) experimental colitis in the rat. Rats were treated with three doses of DNBS: 1. 10, and 20 mg. Inflammation severity was assessed by tissue colonic wet weight, macroscopic evaluation, and tissue myeloperoxidase (MPO) activity. The capacity ofwater-soluble LMWA was assessed by measuring the reducing power of the tissues with cyclic voltammetry (CV) and by measuring ti;sue levels of reducedglutathione. While typical markers of inflammation (MPO, macroscopic examination, and colonic wet weight) indicated DNBS dose dependency, such dependency could not be demonstrated for the tissue LMWA as measured by reduced glutathione levels and by the tissues' reducing power. Mild colonic inflammation (induced by ethanol or by 1 mg of DNBS) caused an increase in the overall capacity of water-soluble LMWA. However, severe inflammation (induced by 20 mg of DNBS) caused a reduction in the tissue LMWA capacity. An intermediate dose of DNBS (10 mg) caused moderate inflammation, but did not cause a significant change in the tissue LMWA compared with a saline control treatment. In conclusion, LMWA changed in a biphasic pattern reflective of the severity of mucosal colonic inflammation. It is suggested that: low dose ofDNBS (1 mg) and topical alcohol (25% v/v) caused an adaptation effect to the mild oxidative stress associated with mild inflammation. This resulted in an increase in the LMWA. A higher dose of DNBS (20 mg) caused more severeinflammation with an overall reduction in LMWA, The increased efflux of reactive oxygen species, associated with severe inflammation, led to an overall consumption of the tissue LMWA, which masked the increase in LMWA causedby the mild oxidative stress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 20:47:14