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Titolo:
Analysis of cranial neural crest migratory pathways in axolotl using cell markers and transplantation
Autore:
Epperlein, HH; Meulemans, D; Bronner-Fraser, M; Steinbeisser, H; Selleck, MAJ;
Indirizzi:
Univ So Calif, Keck Sch Med, Dept Cell & Neurobiol, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 urobiol, Los Angeles, CA 90089 USA Tech Univ Dresden, Inst Anat, D-01307 Dresden, Germany Tech Univ Dresden Dresden Germany D-01307 Anat, D-01307 Dresden, Germany CALTECH, Div Biol, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125CALTECH, Div Biol, Pasadena, CA 91125 USA CALTECH, Beckman Inst, Pasadena, CA 91125 USA CALTECH Pasadena CA USA 91125 LTECH, Beckman Inst, Pasadena, CA 91125 USA Max Planck Inst Entwicklungsbiol, D-72076 Tubingen, Germany Max Planck Inst Entwicklungsbiol Tubingen Germany D-72076 ingen, Germany
Titolo Testata:
DEVELOPMENT
fascicolo: 12, volume: 127, anno: 2000,
pagine: 2751 - 2761
SICI:
0950-1991(200006)127:12<2751:AOCNCM>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR AP-2; VITAL DYE ANALYSIS; AMBYSTOMA-MEXICANUM; REVEALS MULTIPOTENCY; HOX CODE; DIFFERENTIATION; EXPRESSION; EMBRYOGENESIS; CARTILAGE; ROTATION;
Keywords:
DiI; AP-2; cell movement; cartilage differentiation; branchial arches; axolotl; neural crest;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Selleck, MAJ Univ So Calif, Keck Sch Med, Dept Cell & Neurobiol, 1333 San Pablo St,BMT 401, Los Angeles, CA 90089 USA Univ So Calif 1333 San Pablo St,BMT 401 Los Angeles CA USA 90089
Citazione:
H.H. Epperlein et al., "Analysis of cranial neural crest migratory pathways in axolotl using cell markers and transplantation", DEVELOPMENT, 127(12), 2000, pp. 2751-2761

Abstract

We have examined the ability of normal and heterotopically transplanted neural crest cells to migrate along cranial neural crest pathways in the axolotl using focal DiI injections and in situ hybridization with the neural crest marker, AP-2, DiI labeling demonstrates that cranial neural crest cellsmigrate as distinct streams along prescribed pathways to populate the maxillary and mandibular processes of the first branchial arch, the hyoid arch and gill arches 1-4, following migratory pathways similar to those observedin other vertebrates. Another neural crest marker, the transcription factor AP-2, is expressed by premigratory neural crest cells within the neural folds and migrating neural crest cells en route to and within the branchial arches. Rotations of the cranial neural folds suggest that premigratory neural crest cells are not committed to a specific branchial arch fate, but can compensate when displaced short distances from their targets by migratingto a new target arch. In contrast, when cells are displaced far from theiroriginal location, they appear unable to respond appropriately to their new milieu such that they fail to migrate or appear to migrate randomly. Whentrunk neural folds are grafted heterotopically into the head, trunk neuralcrest cells migrate in a highly disorganized fashion and fail to follow normal cranial neural crest pathways. Importantly, we find incorporation of some trunk cells into branchial arch cartilage despite the random nature of their migration. This is the first demonstration that trunk neural crest cells can form cartilage when transplanted to the head. Our results indicate that, although cranial and trunk neural crest cells have inherent differences in ability to recognize migratory pathways, trunk neural crest can differentiate into cranial cartilage when given proper instructive cues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:42:52