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Titolo:
Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60cells: Analysis at the single cell and single organelle level
Autore:
Salvioli, S; Dobrucki, J; Moretti, L; Troiano, L; Fernandez, MG; Pinti, M; Pedrazzi, J; Franceschi, C; Cossarizza, A;
Indirizzi:
Univ Modena & Reggio, Dept Biomed Sci, Chair Immunol, Sect Gen Pathol, I-41100 Modena, Italy Univ Modena & Reggio Modena Italy I-41100 Pathol, I-41100 Modena, Italy Jagiellonian Univ, Lab Confocal Anal & Imaging, Krakow, Poland Jagiellonian Univ Krakow Poland Confocal Anal & Imaging, Krakow, Poland Univ Navarra, Sch Med, Dept Human Physiol, E-31080 Pamplona, Spain Univ Navarra Pamplona Spain E-31080 man Physiol, E-31080 Pamplona, Spain Dept Gerontolog Sci, INRCA, Ancona, Italy Dept Gerontolog Sci Ancona Italy t Gerontolog Sci, INRCA, Ancona, Italy Univ Bologna, Dept Expt Pathol, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 t Expt Pathol, I-40126 Bologna, Italy
Titolo Testata:
CYTOMETRY
fascicolo: 3, volume: 40, anno: 2000,
pagine: 189 - 197
SICI:
0196-4763(20000701)40:3<189:MHDSAI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHLOROMETHYL-X-ROSAMINE; FLOW CYTOMETRIC DETECTION; PHOSPHATIDYLSERINE EXPRESSION; CYTOFLUOROMETRIC ANALYSIS; THYMOCYTE APOPTOSIS; PROTEIN-KINASE; CYTOCHROME-C; ANNEXIN-V; BCL-2; DEATH;
Keywords:
apoptosis; mitochondria; mitochondrial membrane potential; JC-1; MitoTracker; flow cytometry; confocal microscopy; HL60 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Cossarizza, A Univ Modena & Reggio, Dept Biomed Sci, Chair Immunol, Sect Gen Pathol, ViaCampi 287, I-41100 Modena, Italy Univ Modena & Reggio Via Campi 287 Modena Italy I-41100 aly
Citazione:
S. Salvioli et al., "Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60cells: Analysis at the single cell and single organelle level", CYTOMETRY, 40(3), 2000, pp. 189-197

Abstract

Background: Apoptosis is a complex phenomenon during which several events occur. A growing interest exists on the role and functionality of mitochondria during this type of cell death. The responsibility of modifications in mitochondrial membrane potential (Delta Psi) in triggering apoptosis is under investigation. Methods: We evaluated Delta Psi changes in HL60 cells treated with staurosporine (STS). Flow cytometry and confocal microscopy have been used to analyze samples stained with two Delta Psi-sensitive probes, JC1 and MitoTracker(TM) Red CMXRos. Results: At the cellular level, we found heterogeneic behavior. Indeed, after STS treatment, some cells displayed typical markers of apoptosis and a collapse in Delta Psi. Others were apoptotic with no changes in Delta Psi, others changed Delta Psi without being apoptotic, and others were healthy. The same heterogeneic response to STS was found at the single organelle level. In a given cell, some mitochondria were depolarized whereas others werenot. Conclusion: In this model of apoptosis, changes in Delta Psi can be different among cells of the same type and among different organelles of the samecell. The collapse in Delta Psi is thus a heterogeneic phenomenon that seems to be an ancillary event following the irreversible phase of the apoptotic process. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 07:56:14