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Titolo:
Comparison of two recombinant major outer membrane proteins of the human granulocytic ehrlichiosis agent for use in an enzyme-linked immunosorbent assay
Autore:
Tajima, T; Zhi, N; Lin, Q; Rikishia, Y; Horowitz, HW; Ralfalli, J; Wormser, GP; Hechemy, KE;
Indirizzi:
Ohio State Univ, Coll Vet Med, Dept Vet Biosci, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 Vet Biosci, Columbus, OH 43210 USA New York Med Coll, Dept Med, Div Infect Dis, Valhalla, NY 10595 USA New York Med Coll Valhalla NY USA 10595 nfect Dis, Valhalla, NY 10595 USA New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA New York State Dept Hlth Albany NY USA 12201 th Ctr, Albany, NY 12201 USA
Titolo Testata:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
fascicolo: 4, volume: 7, anno: 2000,
pagine: 652 - 657
SICI:
1071-412X(200007)7:4<652:COTRMO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TICK-BORNE PATHOGENS; NEW-YORK-STATE; BORRELIA-BURGDORFERI; LYME-DISEASE; HUMAN SERA; ANTIBODIES; SERODIAGNOSIS; COINFECTION; WESTERN; EUROPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Rikishia, Y Ohio State Univ, Coll Vet Med, Dept Vet Biosci, 1925 Coffey Rd, Columbus, OH 43210 USA Ohio State Univ 1925 Coffey Rd Columbus OH USA 43210 43210 USA
Citazione:
T. Tajima et al., "Comparison of two recombinant major outer membrane proteins of the human granulocytic ehrlichiosis agent for use in an enzyme-linked immunosorbent assay", CL DIAG LAB, 7(4), 2000, pp. 652-657

Abstract

Enzyme-linked immunosorbent assay (ELISA) for human granulocytic ehrlichiosis (HGE) using two different recombinant p44 proteins (rP-44 and rP-44-2hv) of the HGE agent as antigens was evaluated. Sera from a total of 72 healthy humans both from regions where HGE is nonendemic and regions where HGE is endemic were used as negative controls to determine the cutoff value for ELISA. Sera from a total of 14 patients (nine from whom the HGE agent was isolated and five who were HGE-PCR positive) were used as positive controls. One hundred nine sera from 72 patients in an area where HGE is endemic whowere suspected of having HGE were examined by ELISA and indirect immunofluorescence assay (IFA). All LFA-negative sera were negative by both ELISAs. Of 39 sera that mere IFA positive, 35 and 27 were positive by ELISA using rP44 and rP44-2hv, respectively, indicating that the use of rP44 is more sensitive. Western blot analysis of the four rP44-ELISA-negative IFA-positive sera using whole HGE agent as antigen suggests that these four sera were false IFA positive. There was no difference in results with or without the preabsorption of sera with Escherichia coli or with or without the cleavage of the fused protein derived from the vector. There was a significant positive correlation between IFA titers and optical densities of ELISAs. Four Ehrlichia chaffeensis-positive and 10 Borrelia burgdorferi-positive sera were negative by ELISA. However, two Babesia microti-positive sera showed strongcross-reactivity to the Fused vector protein, which was eliminated after cleavage of the protein. Thus, ELISA using rP44 nonfusion protein would provide a simple, specific, and objective HGE serologic test which can be easily automated.

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Documento generato il 15/07/20 alle ore 05:26:55