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Titolo:
Vascular endothelial growth factor(165) gene transfer augments circulatingendothelial progenitor cells in human subjects
Autore:
Kalka, C; Masuda, H; Takahashi, T; Gordon, R; Tepper, O; Gravereaux, E; Pieczek, A; Iwaguro, H; Hayashi, SI; Isner, JM; Asahara, T;
Indirizzi:
St Elizabeths Med Ctr, Tufts Sch Med, Dept Med Cardiol, Boston, MA 02135 USA St Elizabeths Med Ctr Boston MA USA 02135 d Cardiol, Boston, MA 02135 USA St Elizabeths Med Ctr, Tufts Sch Med, Dept Biomed Res, Boston, MA 02135 USA St Elizabeths Med Ctr Boston MA USA 02135 iomed Res, Boston, MA 02135 USA
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 12, volume: 86, anno: 2000,
pagine: 1198 - 1202
SICI:
0009-7330(20000623)86:12<1198:VEGFGT>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANGIOGENESIS; DISEASE; NEOVASCULARIZATION; VASCULOGENESIS; PHVEGF(165); EXPRESSION; MECHANISMS; ISCHEMIA; THERAPY; FLK-1;
Keywords:
vascular endothelial growth factor; gene therapy; endothelial progenitor cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Isner, JM St Elizabeths Med Ctr, Tufts Sch Med, Dept Med Cardiol, 736 Cambridge St, Boston, MA 02135 USA St Elizabeths Med Ctr 736 Cambridge St Boston MA USA 02135 5 USA
Citazione:
C. Kalka et al., "Vascular endothelial growth factor(165) gene transfer augments circulatingendothelial progenitor cells in human subjects", CIRCUL RES, 86(12), 2000, pp. 1198-1202

Abstract

Preclinical studies in animal models and early results of clinical trials in patients suggest that intramuscular injection of naked plasmid DNA encoding vascular endothelial growth factor (VEGF) can promote neovascularization of ischemic tissues. Such neovascularization has been attributed exclusively to sprout formation of endothelial cells derived from preexisting vessels. We investigated the hypothesis that VEGF gene transfer may also augmentthe population of circulating endothelial progenitor cells (EPCs). In patients with critical limb ischemia receiving VEGF gene transfer, gene expression was documented by a transient increase in plasma levels of VEGF. A culture assay documented a significant increase in EPCs (219%, P < 0.001), whereas patients who received an empty vector had no change in circulating EPCs, as was the case for volunteers who received saline injections (VEGF versus empty vector, P < 0.001; VEGF versus saline, P < 0.005). Fluorescence-activated cell sorter analysis disclosed an overall increase of up to 30-fold in endothelial lineage markers KDR (VEGF receptor-2), VE-cadherin, CD34, alpha(v)beta(3), and E-selectin after VEGF gene transfer. Constitutive overexpression of VEGF in patients with limb ischemia augments the DnDUlation of circulating EPCs. These findings support the notion that neovascularizationof human ischemic tissues after angiogenic growth factor therapy is not limited to angiogenesis but involves circulating endothelial precursors that may home to ischemic foci and differentiate in situ through a process of vasculogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 12:25:12