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Titolo:
The 300-kDa intermediate filament-associated protein (IFAP300) is a hamster plectin ortholog
Autore:
Clubb, BH; Chou, YH; Herrmann, H; Svitkina, TM; Borisy, GG; Goldman, RD;
Indirizzi:
Northwestern Univ, Sch Med, Dept Cell & Mol Biol, Chicago, IL 60657 USA Northwestern Univ Chicago IL USA 60657 & Mol Biol, Chicago, IL 60657 USA German Canc Res Ctr, Div Cell Biol, D-6900 Heidelberg, Germany German CancRes Ctr Heidelberg Germany D-6900 D-6900 Heidelberg, Germany Univ Wisconsin, Mol Biol Lab, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 , Mol Biol Lab, Madison, WI 53706 USA
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 273, anno: 2000,
pagine: 183 - 187
SICI:
0006-291X(20000624)273:1<183:T3IFP(>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMOLYSIS-BULLOSA SIMPLEX; MUSCULAR-DYSTROPHY; CULTURED-CELLS; CYTOSKELETON; VIMENTIN; ORGANIZATION; ANTIBODIES; JUNCTIONS; BINDING; SUBUNIT;
Keywords:
cytoskeleton; plectin; intermediate filament-associated protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Goldman, RD Northwestern Univ, Sch Med, Dept Cell & Mol Biol, 303 E Chicago Ave, Chicago, IL 60657 USA Northwestern Univ 303 E Chicago Ave Chicago ILUSA 60657 7 USA
Citazione:
B.H. Clubb et al., "The 300-kDa intermediate filament-associated protein (IFAP300) is a hamster plectin ortholog", BIOC BIOP R, 273(1), 2000, pp. 183-187

Abstract

Plectin is a high-molecular-weight cytoskeleton-associated protein that was initially identified in intermediate filament (IF) enriched fractions of rat C6 glioma cells, At the cellular level, plectin has been found to associate with IF networks and IF-associated structures that are involved in cell-cell and cell-substrate adhesions, IFAP300 is an IF-associated protein that was initially identified in hamster cells by a monoclonal antibody directed against a high molecular weight protein present in IF-enriched cytoskeletal preparations. Plectin and IFAP300 display similar distribution patterns within cells as determined by immunofluorescence. Based upon this and thefinding that their biochemical properties are similar, it has been suggested that they may actually be orthologous proteins. In this paper we demonstrate that this is the case. Cloning and sequencing of most of the hamster plectin cDNA demonstrates that plectin is found in hamster cells and that its sequence is highly conserved between species. Using immunological crossreactivity, epitope mapping, and immunoelectron microscopy, we show that IFAP300 is actually the hamster ortholog of plectin. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 09:37:30