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Titolo:
Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing beta-lactamases in Chryseobacterium meningosepticum
Autore:
Bellais, S; Aubert, D; Naas, T; Nordmann, P;
Indirizzi:
Hop Bicetre, Serv Bacteriol Virol, Fac Med Paris Sud, Assistance Publ Hop Paris, F-94275 Le Kremlin Bicetre, France Hop Bicetre Le Kremlin Bicetre France F-94275 Le Kremlin Bicetre, France
Titolo Testata:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
fascicolo: 7, volume: 44, anno: 2000,
pagine: 1878 - 1886
SICI:
0066-4804(200007)44:7<1878:MABHOC>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS-AERUGINOSA; FLAVOBACTERIUM-MENINGOSEPTICUM; BACTEROIDES-FRAGILIS; ESCHERICHIA-COLI; GENE BLA(IMP); STENOTROPHOMONAS-MALTOPHILIA; SERRATIA-MARCESCENS; CRYSTAL-STRUCTURE; SEQUENCE-ANALYSIS; CLINICAL ISOLATE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Nordmann, P Hop Bicetre, Serv Bacteriol Virol, Fac Med Paris Sud, Assistance Publ Hop Paris, 78 Rue Gen Leclerc, F-94275 Le Kremlin Bicetre, France Hop Bicetre 78 Rue Gen Leclerc Le Kremlin Bicetre France F-94275
Citazione:
S. Bellais et al., "Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing beta-lactamases in Chryseobacterium meningosepticum", ANTIM AG CH, 44(7), 2000, pp. 1878-1886

Abstract

Although the carbapenem-hydrolyzing beta-lactamase (CH beta L) BlaB-1 is known to be in Chryseobacterium meningosepticum NCTC 10585, a second CH betaL gene, bla(GOB-1) was cloned from another C, meningosepticum clinical isolate (PINT). The G+C content of bla(GOB-1) (36%) indicated the likely chromosomal origin of this gene. Its expression in Escherichia coli DH10B yieldsa mature CH beta L with a pI of 8.7 and a relative molecular mass of 28.2 kDa, In E, coli, GOB-1 conferred resistance to narrow-spectrum cephalosporins and reduced susceptibility to ureidopenicillins, broad-spectrum cephalosporins, and carbapenems, GOB-1 had a broad-spectrum hydrolysis profile including penicillins and cephalosporins (but not aztreonam), The catalytic efficiency for meropenem was higher than for imipenem, GOB-1 had low amino acid identity with the class B CH beta Ls, sharing 18% with the closest, L-1 from Stenotrophomonas maltophilia, and only 11% with BlaB-1, h-Post of the conserved amino acids that may be involved in the active site of CH beta Ls (His-101, Asp-103, His-162, and His-225) were identified in GOB-1, Sequenceheterogeneity was found for GOB-1-like and BlaB-1-like beta-lactamases, having 90 to 100% and 86 to 100% amino acid identity, respectively, among 10 unrelated C. meningosepticum isolates. Each isolate had a GOB-1-like and a BlaB-1-like gene. The same combination of GOB-1-like and BlaB-1-like beta-lactamases was not found in two different isolates. C. meningosepticum is a bacterial species with two types of unrelated chromosome-borne class E CH beta Ls that can be expressed in E, coil and, thus, may represent a clinicalthreat if spread in gram-negative aerobes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 23:09:14