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Titolo:
Analysis of ternary mixtures with a single dynamic microbial sensor and chemometrics using a nonlinear multivariate calibration
Autore:
Plegge, V; Slama, M; Suselbeck, B; Wienke, D; Spener, F; Knoll, M; Zaborosch, C;
Indirizzi:
Inst Chemo & Biosensorik, D-48149 Munster, Germany Inst Chemo & Biosensorik Munster Germany D-48149 -48149 Munster, Germany Univ Munster, Zentrum Informat Arbeitung, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 Arbeitung, D-48149 Munster, Germany DSM Res, NL-6160 MD Geleen, Netherlands DSM Res Geleen Netherlands NL-6160 MD es, NL-6160 MD Geleen, Netherlands Univ Munster, Inst Biochem, D-48149 Munster, Germany Univ Munster Munster Germany D-48149 t Biochem, D-48149 Munster, Germany
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 13, volume: 72, anno: 2000,
pagine: 2937 - 2942
SICI:
0003-2700(20000701)72:13<2937:AOTMWA>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLOW-INJECTION-ANALYSIS; SELECTIVE ELECTRODE ARRAYS; MULTICOMPONENT ANALYSIS; CURVE RESOLUTION; ORGANIC VAPORS; AMINO-ACIDS; REGRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Zaborosch, C Inst Chemo & Biosensorik, Mendelstr 7, D-48149 Munster, Germany Inst Chemo & Biosensorik Mendelstr 7 Munster Germany D-48149
Citazione:
V. Plegge et al., "Analysis of ternary mixtures with a single dynamic microbial sensor and chemometrics using a nonlinear multivariate calibration", ANALYT CHEM, 72(13), 2000, pp. 2937-2942

Abstract

An amperometric biosensor based on immobilized bacterial cells of Alcaligenes eutrophus KT02 and an oxygen electrode was integrated in a now-through system. Because microorganisms metabolize various organic analytes in a specific manner, the sensor shows for different pure analytes distinct time-dependent oxygen consumption rates that can be treated as characteristic patterns. This behavior is conserved also when the biosensor is exposed to a mixture of these organic analytes; the sensor with a particular type of microorganisms responds with a total signal. The respiration curves as time-dependent amplitudes were subdivided into several time channels. This procedurecreates an additional data dimension and makes the single sensor "dynamic". Using multivariate calibration models with only one single biosensor, simultaneous quantitative analysis of ternary mixtures of acetate, L-lactate, and succinate was realized. A nonlinear algorithm that compensated for conceivable interactions of the analytes was superior to a partial least-squares algorithm. Each analyte was predicted more precisely by the nonlinear approach resulting in root-mean-square errors of prediction of 0.20 mg/L for acetate, 0.43 mg/L for L-lactate, and 0.73 mg/L for succinate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:07:18