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Titolo:
Sequence analysis of Pns11, a nonstructural protein of rice gall dwarf virus, and its expression and detection in infected rice plants and vector insects
Autore:
Moriyasu, Y; Ishikawa, K; Kikuchi, A; Imanishi, S; Tomita, S; Akutsu, K; Omura, T;
Indirizzi:
Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan Natl Agr Res Ctr Tsukuba Ibaraki Japan 3058666 ba, Ibaraki 3058666, Japan Natl Inst Sericultural & Entomol Sci, Tsukuba, Ibaraki 3058634, Japan NatlInst Sericultural & Entomol Sci Tsukuba Ibaraki Japan 3058634 Japan Ibaraki Univ, Fac Agr, Ibaraki, Osaka 3000332, Japan Ibaraki Univ IbarakiOsaka Japan 3000332 r, Ibaraki, Osaka 3000332, Japan
Titolo Testata:
VIRUS GENES
fascicolo: 3, volume: 20, anno: 2000,
pagine: 237 - 241
SICI:
0920-8569(200005)20:3<237:SAOPAN>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE; CAPSID PROTEIN; GENOME; PHYTOREOVIRUS; PARTICLES; S10; P2;
Keywords:
RGDV; Phytoreovirus; Pns11;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Omura, T Natl Agr Res Ctr, Tsukuba, Ibaraki 3058666, Japan Natl Agr Res Ctr Tsukuba Ibaraki Japan 3058666 ki 3058666, Japan
Citazione:
Y. Moriyasu et al., "Sequence analysis of Pns11, a nonstructural protein of rice gall dwarf virus, and its expression and detection in infected rice plants and vector insects", VIRUS GENES, 20(3), 2000, pp. 237-241

Abstract

The nucleotide sequence of genome segment S11 of rice gall dwarf virus (RGDV), a member of Phytoreovirus, was determined. The segment encodes a putative protein of 40 kDa that exhibits approximately 37% homology at the aminoacid level to the nonstructural proteins Pns10 of rice dwarf and wound tumor viruses, which are other members of Phytoreovirus. A band of a protein with an apparent molecular mass of 40 kDa was specifically detected in an analysis of cells transfected with S11 cDNA. An antiserum raised against thisprotein reacted with a protein of approximately 40 kDa after fractionationby SDS-PAGE of materials prepared from infected plants and from viruliferous vector insects. However, the antiserum did not react with purified viralproteins. These results suggest that S11 encodes a nonstructural protein of RGDV. This protein was named Pns11.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:30:33