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Titolo:
In vivo detection of amyloid plaques in a mouse model of Alzheimer's disease
Autore:
Skovronsky, DM; Zhang, B; Kung, MP; Kung, HF; Trojanowski, JQ; Lee, VMY;
Indirizzi:
Univ Penn, Sch Med, Ctr Neurodegenerat Dis Res, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 & Lab Med, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Ctr Neurodegenerat Dis Res, Dept Radiol, Philadelphia,PA 19104 USA Univ Penn Philadelphia PA USA 19104 ept Radiol, Philadelphia,PA 19104 USA Univ Penn, Sch Med, Ctr Neurodegenerat Dis Res, Dept Pharmacol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 Pharmacol, Philadelphia, PA 19104 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 13, volume: 97, anno: 2000,
pagine: 7609 - 7614
SICI:
0027-8424(20000620)97:13<7609:IVDOAP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BETA-PROTEIN-PRECURSOR; TRANSGENIC MICE; IN-VIVO; PRESENILIN-1; CELLS; ACCUMULATION; DEGRADATION; A-BETA-1-42; RESISTANCE; PROBE;
Keywords:
amyloid beta-protein; imaging; senile plaques;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, VMY Univ Penn, Sch Med, Ctr Neurodegenerat Dis Res, Dept Pathol & LabMed, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 , Philadelphia, PA 19104 USA
Citazione:
D.M. Skovronsky et al., "In vivo detection of amyloid plaques in a mouse model of Alzheimer's disease", P NAS US, 97(13), 2000, pp. 7609-7614

Abstract

Strategies for treating Alzheimer's disease (AD) include therapies designed to decrease senile plaque (SP) formation and/or promote clearance of SPs.but clinical trials of these treatments are limited by the lack of effective methods to monitor changes in plaque burden in the brains of living AD patients. However. because SPs are extracellular deposits of amyloid-beta peptides (A beta), it may be possible to eventually develop radioligands thatcross the blood-brain barrier (BBB) and label SPs so they can be visualized by current imaging methods. As a first step toward the generation of sucha radioligand, we developed a probe, [(trans,trans)-1-bromo-2,5-bis-(3-hydroxycarbonyl-4-hydroxy)styrylbenzene(BSB)], and we report here that BSB hasthe following properties essential for a probe that can detect SPs in vivo, First, BSB sensitively labels SPs in AD brain sections. Second. BSB permeates living cells in culture and binds specifically to intracellular A betaaggregates. Third, after intracerebral injection in living transgenic mouse models of AD amyloidosis. BSB labels SPs composed of human A beta with high sensitivity and specificity. fourth, BSB crosses the BBB and labels numerous AD-like SPs throughout the brain of the transgenic mice after i.v. injection. Thus, we conclude that BSB is an appropriate starting point for future efforts to generate an antemortem diagnostic for AD.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 04:52:49