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Titolo:
SURVIVAL OF TRYPANOSOMA-BRUCEI IN THE TSETSE-FLY IS ENHANCED BY THE EXPRESSION OF SPECIFIC FORMS OF PROCYCLIN
Autore:
RUEPP S; FURGER A; KURATH U; RENGGLI CK; HEMPHILL A; BRUN R; RODITI I;
Indirizzi:
UNIV BERN,INST ALLGEMEINE MIKROBIOL,BALTZERSTR 4 CH-3012 BERN SWITZERLAND UNIV BERN,INST ALLGEMEINE MIKROBIOL CH-3012 BERN SWITZERLAND SCHWEIZER TROPENINST CH-4051 BASEL SWITZERLAND UNIV BERN,INST PARASITOL CH-3012 BERN SWITZERLAND
Titolo Testata:
The Journal of cell biology
fascicolo: 6, volume: 137, anno: 1997,
pagine: 1369 - 1379
SICI:
0021-9525(1997)137:6<1369:SOTITT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACIDIC REPETITIVE PROTEIN; AMANITIN-RESISTANT TRANSCRIPTION; VARIANT SURFACE GLYCOPROTEIN; SELECTABLE MARKER; AFRICAN TRYPANOSOMES; BLOOD-STREAM; MESSENGER-RNAS; CULTURE FORMS; PARP PROMOTER; GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
S. Ruepp et al., "SURVIVAL OF TRYPANOSOMA-BRUCEI IN THE TSETSE-FLY IS ENHANCED BY THE EXPRESSION OF SPECIFIC FORMS OF PROCYCLIN", The Journal of cell biology, 137(6), 1997, pp. 1369-1379

Abstract

African trypanosomes are not passively transmitted, but they undergo several rounds of differentiation and proliferation within their intermediate host, the tsetse fly. At each stage, the survival and successful replication of the parasites improve their chances of continuing the life cycle, but little is known about specific molecules that contribute to these processes. Procyclins are the major surface glycoproteins of the insect forms of Trypanosoma brucei. Six genes encode proteinswith extensive glutamic acid-proline dipeptide repeats (EP in the single-letter amino acid code), and two genes encode proteins with an internal pentapeptide repeat (GPEET). To study the function of procyclins, we have generated mutants that have no EP genes and only one copy ofGPEET. This last gene could not be replaced by EP procyclins, and could only be deleted once a second GPEET copy was introduced into another locus. The EP knockouts are morphologically indistinguishable from the parental strain, but their ability to establish a heavy infection in the insect midgut is severely compromised; this phenotype can be reversed by the reintroduction of a single, highly expressed EP gene. These results suggest that the two types of procyclin have different roles, and that the EP form, while not required in culture, is important for survival in the fly.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:08:34