Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Reporter constructs showing proper globin gene switching in cultured erythroid cell lines
Autore:
Kim, NG; Chae, JH; Choe, SY; Kim, CG;
Indirizzi:
Hanyang Univ, Coll Nat Sci, Dept Life Sci, Seoul 133791, South Korea Hanyang Univ Seoul South Korea 133791 ife Sci, Seoul 133791, South Korea Chungbuk Natl Univ, Fac Life Sci, Chungju 361763, South Korea Chungbuk Natl Univ Chungju South Korea 361763 hungju 361763, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 3, volume: 10, anno: 2000,
pagine: 281 - 288
SICI:
1016-8478(20000630)10:3<281:RCSPGG>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOCUS-CONTROL REGION; HUMAN GAMMA-GLOBIN; KRUPPEL-LIKE FACTOR; BETA-GLOBIN; TRANSGENIC MICE; DEVELOPMENTAL CONTROL; ACTIVATION REGION; REGULATED EXPRESSION; FETAL; HYBRIDS;
Keywords:
erythroid cell lines; globin gene switching; regulatory elements; reporter constructs;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, CG Hanyang Univ, Coll Nat Sci, Dept Life Sci, Seoul 133791, South Korea Hanyang Univ Seoul South Korea 133791 Seoul 133791, South Korea
Citazione:
N.G. Kim et al., "Reporter constructs showing proper globin gene switching in cultured erythroid cell lines", MOL CELLS, 10(3), 2000, pp. 281-288

Abstract

Recent advances in the study of globin gene switching in the context of complete gene locus have contributed greatly to our understanding of developmental regulation mechanism of globin gene expression. However, it is not clear yet whether the cluster is sufficient in proper gene switching when theglobin genes are replaced with conventional reporter genes. Furthermore, even though erythroid-specific and ubiquitous transcription factors involvedin erythroid-specific globin gene expression have been characterized and some plausible globin gene switching models have been suggested, any specific factor directly involved in globin gene switching is not yet identified. In this study, as an effort to further understand globin switching mechanism and to identify globin switching factors, we constructed reporter vectorsby juxtaposing several putative regulatory elements in human beta-globin locus to conventional reporter genes and analyzed their stage-specific expression in erythroid cell lines. At the end, we demonstrated that gamma beta-type constructs, in which both gamma-type and beta-type globin reporter genes were linked in cis below beta-globin locus control region (LCR), show proper stage-specific reporter gene expression in erythroid cell lines and also recapitulate globin switching in cell hybrids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:19:55