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Titolo:
Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase (TPS1) gene from Saccharomyces cerevisiae
Autore:
Yeo, ET; Kwon, HB; Han, SE; Lee, JT; Ryu, JC; Byun, MO;
Indirizzi:
Natl Inst Agr Sci & Technol, Div Mol Genet, Suwon 441707, South Korea NatlInst Agr Sci & Technol Suwon South Korea 441707 441707, South Korea Kyungpook Natl Univ, Dept Agr Biol, Taegu 702701, South Korea Kyungpook Natl Univ Taegu South Korea 702701 , Taegu 702701, South Korea Sunmoon Univ, Div Appl Biol Sci, Asan 336840, South Korea Sunmoon Univ Asan South Korea 336840 Biol Sci, Asan 336840, South Korea
Titolo Testata:
MOLECULES AND CELLS
fascicolo: 3, volume: 10, anno: 2000,
pagine: 263 - 268
SICI:
1016-8478(20000630)10:3<263:GEODRP>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TREHALOSE SYNTHASE; TRANSGENIC PLANTS; YEAST; TOLERANCE; TOBACCO; EXPRESSION; GLYCOLYSIS; CLONING; GLUCOSE; CIF1;
Keywords:
drought tolerance; Saccharomyces cerevisiae; transgenic potato plant; trehalose; trehalose-6-phosphate synthase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Byun, MO Natl Inst Agr Sci & Technol, Div Mol Genet, Suwon 441707, South Korea Natl Inst Agr Sci & Technol Suwon South Korea 441707 outh Korea
Citazione:
E.T. Yeo et al., "Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase (TPS1) gene from Saccharomyces cerevisiae", MOL CELLS, 10(3), 2000, pp. 263-268

Abstract

In yeast, trehalose-6-phosphate synthase is a key enzyme for trehalose biosynthesis, encoded by the structural gene TPS1, Trehalose affects sugar metabolism as well as osmoprotection against several environmental stresses, such as heat and desiccation, The TPS1 gene of Saccharomyces cerevisiae was engineered under the control of the CaMV 35S promoter for constitutive expression in transgenic potato plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting TPS1 transgenic potato plants exhibited various morphological phenotypes in culture tubes, ranging from normal to severely retarded growth, including dwarfish growth, yellowish lancet-shaped leaves, and aberrant root development. However, the plants recovered from thesenegative growth effects when grown in a soil mixture. The TPS1 transgenic potato plants showed significantly increased drought resistance. These results suggest that the production of trehalose not only affects plant development but also improves drought tolerance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 13:54:10