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Titolo:
Retinal degeneration in cone photoreceptor cell-ablated transgenic mice
Autore:
Ying, SX; Jansen, HT; Lehman, MN; Fong, SL; Kao, WWY;
Indirizzi:
Univ Cincinnati, Dept Ophthalmol, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 thalmol, Cincinnati, OH 45267 USA Univ Cincinnati, Dept Cell Biol Neurobiol & Anat, Cincinnati, OH 45267 USAUniv Cincinnati Cincinnati OH USA 45267 & Anat, Cincinnati, OH 45267 USA Indiana Univ, Dept Ophthalmol, Indianapolis, IN 46204 USA Indiana Univ Indianapolis IN USA 46204 halmol, Indianapolis, IN 46204 USA
Titolo Testata:
MOLECULAR VISION
fascicolo: 12, volume: 6, anno: 2000,
pagine: 101 - 108
SICI:
1090-0535(20000624)6:12<101:RDICPC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURON-SPECIFIC ENOLASE; TOXIN-A-CHAIN; DIPHTHERIA-TOXIN; MOUSE RETINA; TRANSFECTED CELLS; SURVIVAL FACTOR; EXPRESSION; PIGMENTOSA; EPITHELIUM; LINEAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kao, WWY Univ Cincinnati, Dept Ophthalmol, Eden & Bethesda Ave,ML 527, Cincinnati, OH 45267 USA Univ Cincinnati Eden & Bethesda Ave,ML 527 CincinnatiOH USA 45267
Citazione:
S.X. Ying et al., "Retinal degeneration in cone photoreceptor cell-ablated transgenic mice", MOL VIS, 6(12), 2000, pp. 101-108

Abstract

PURPOSE: To examine the effect of loss of cone photoreceptor cells on retinal degeneration. METHODS: We previously identified a cone photoreceptor cell-specific promoter of human cone transducin alpha-subunit (GNAT2) gene. In this report, a minigene, Trc-Tox176, that contains the GNAT2 promoter, an attenuated diphtheria toxin A-chain gene, and an enhancer element from human interphotoreceptor retinoid-binding protein (IRBP) was used to generate coneless transgenic mice. Transgenic mice were identified by PCR and the copy number of the transgene was determined by Southern hybridization, and examined by histology. RESULTS: The results of immunostaining with anti-mouse GNAT2 antibodies and reverse transcription-PCR (RT-PCR) analysis with mRNA from the retinas oftransgenic mice showed that cone photoreceptor cells were ablated in one of four transgenic mouse lines. The ablation of cone cells began at postnatal day 8, at the same time as the expression of endogenous GNAT2. An age-related rod degeneration was also found in this cone-ablated mouse line, beginning at postnatal day 9, proceeding from the central retina to the peripheral retina. CONCLUSIONS: Cone photoreceptor cells may play an important role in the survival of rod photoreceptor cells during mouse retina development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:11:49