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Titolo:
A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression
Autore:
Li, HO; Zhu, YF; Asakawa, M; Kuma, H; Hirata, T; Ueda, Y; Lee, YS; Fukumura, M; Iida, A; Kato, A; Nagai, Y; Hasegawa, M;
Indirizzi:
DNAVEC Res Inc, Tsukuba, Ibaraki 3050856, Japan DNAVEC Res Inc Tsukuba Ibaraki Japan 3050856 kuba, Ibaraki 3050856, Japan Univ Tokyo, Inst Med Sci, Dept Viral Infect, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 l Infect, Minato Ku, Tokyo 1088639, Japan
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 14, volume: 74, anno: 2000,
pagine: 6564 - 6569
SICI:
0022-538X(200007)74:14<6564:ACRVDF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR STOMATITIS VIRUSES; MEASLES-VIRUS; FOREIGN GENE; CRE RECOMBINASE; REPORTER GENE; CELL-FUSION; PROTEIN; SYSTEM; RESCUE; PROPAGATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Iida, A DNAVEC Res Inc, 1-25-11 Kannondai, Tsukuba, Ibaraki 3050856, JapanDNAVEC Res Inc 1-25-11 Kannondai Tsukuba Ibaraki Japan 3050856 pan
Citazione:
H.O. Li et al., "A cytoplasmic RNA vector derived from nontransmissible Sendai virus with efficient gene transfer and expression", J VIROLOGY, 74(14), 2000, pp. 6564-6569

Abstract

We have recovered a virion from defective cDNA of Sendai virus (SeV) that is capable of self-replication but incapable of transmissible-virion production. This virion delivers and expresses foreign genes in infected cells, and this is the first report of a gene expression vector derived from a defective viral genome of the Paramyxoviridae, First, functional ribonucleoprotein complexes (RNPs) were recovered from SeV cloned cDNA defective in the F(envelope fusion protein) gene, in the presence of plasmids expressing nucleocapsid protein and viral RNA polymerase. Then the RNPs were transfected to the cells inducibly expressing F protein. Virion-like particles thus obtained had a titer of 0.5 x 10(8) to 1.0 x 10(8) cell infectious units/ml and contained F defective RNA genome. This defective vector amplified specifically in an F expressing packaging cell line in a trypsin-dependent manner but did not spread to F-nonexpressing cells. This vector infected and expressed an enhanced green fluorescent protein reporter gene in various types of animal and human cells, including nondividing cells, with high efficiency. These results suggest that this vector has great potential for use in human gene therapy and vaccine delivery systems.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:23:56