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Titolo:
Chromatographic determination of riboflavin and its derivatives in food
Autore:
Gliszczynska-Swiglo, A; Koziolowa, A;
Indirizzi:
Poznan Univ Econ, Fac Commod Sci, PL-60967 Poznan, Poland Poznan Univ Econ Poznan Poland PL-60967 mod Sci, PL-60967 Poznan, Poland
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 881, anno: 2000,
pagine: 285 - 297
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; FLAVIN DERIVATIVES; RAT URINE; MILK; IDENTIFICATION; LIVER; GLUCOSIDE; HPLC; 7-ALPHA-HYDROXYRIBOFLAVIN; QUANTITATION;
Keywords:
food analysis; riboflavins; vitamins; flavins; nucleotides; glycosides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
63
Recensione:
Indirizzi per estratti:
Indirizzo: Koziolowa, A Poznan Univ Econ, Fac Commod Sci, Al Niepodleglosci 10, PL-60967 Poznan, Poland Poznan Univ Econ Al Niepodleglosci 10 Poznan Poland PL-60967
Citazione:
A. Gliszczynska-Swiglo e A. Koziolowa, "Chromatographic determination of riboflavin and its derivatives in food", J CHROMAT A, 881(1-2), 2000, pp. 285-297

Abstract

Three elution methods on two different reversed-phase C-18 columns were developed to determine flavin(1) derivatives in raw egg white, raw egg yolk, egg powder, pasteurised milk, fermented milk products and liver (chicken, calf and pig). Additionally, 11 thin-layer chromatography solvent systems were used to confirm presence of flavins detected in assessed products. It was found that an Alphabond C-18 column was not as effective as a Symmetry C-18 column. Method A (mobile phase gradient of methanol-0.05 M ammonium acetate, pH 6.0 applied on an Alphabond C-18 column) can be used for determination of flavin adenine dinucleotide, flavin mononucleotide, riboflavin 4',5'-cyclic phosphate, riboflavin, 10-formylmethylflavin and 10-hydroxyethylflavin in products that do not contain 7 alpha-hydroxyriboflavin. Method B (mobile phase gradient of methanol-demineralized water, on an Alphabond C-18 column) can be useful to separate flavin coenzymes from other flavin compounds or to confirm the presence of 7 alpha-hydroxyriboflavin and 10-hydroxyethylflavin in analysed samples. Method C (mobile phase gradient of methanol-0.05 M ammonium acetate, pH 6.0, on a Symmetry C-18 column) allows separation of all flavins detected in tested products: flavin adenine dinucleotide,flavin mononucleotide, riboflavin 4',5'-cyclic phosphate, riboflavin, 10-formylmethylflavin, 10-hydroxyethylflavin, 7 alpha-hydroxyriboflavin, riboflavin-beta-D-galactoside and riboflavin-alpha-D-glucoside. (C) 2000 ElsevierScience BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 21:14:18