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Titolo:
Simultaneous determination of triclabendazole and its sulphoxide and sulphone metabolites in bovine milk by high-performance liquid chromatography
Autore:
Takeba, K; Fujinuma, K; Sakamoto, M; Miyazaki, T; Oka, H; Itoh, Y; Nakazawa, H;
Indirizzi:
Tokyo Metropolitan Res Lab Publ Hlth, Shinjuku Ku, Tokyo 1690073, Japan Tokyo Metropolitan Res Lab Publ Hlth Tokyo Japan 1690073 1690073, Japan Aichi Prefectural Inst Publ Hlth, Kita Ku, Nagoya, Aichi 4620032, Japan Aichi Prefectural Inst Publ Hlth Nagoya Aichi Japan 4620032 620032, Japan Hoshi Univ, Shinagawa Ku, Tokyo 1420063, Japan Hoshi Univ Tokyo Japan 1420063 Univ, Shinagawa Ku, Tokyo 1420063, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 882, anno: 2000,
pagine: 99 - 107
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA; SHEEP; FENBENDAZOLE;
Keywords:
milk; food analysis; triclabendazole;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Takeba, K Tokyo Metropolitan Res Lab Publ Hlth, Shinjuku Ku, 3-24-1 Hyakunin Cho, Tokyo 1690073, Japan Tokyo Metropolitan Res Lab Publ Hlth 3-24-1 Hyakunin Cho Tokyo Japan 1690073
Citazione:
K. Takeba et al., "Simultaneous determination of triclabendazole and its sulphoxide and sulphone metabolites in bovine milk by high-performance liquid chromatography", J CHROMAT A, 882(1-2), 2000, pp. 99-107

Abstract

A simple method has been developed fur the simultaneous determination of triclabendazole and its metabolites (sulphoxide and sulphone) in bovine milkby reversed-phase high-performance liquid chromatography (HPLC). A milk sample was homogenized with sodium sulfate anhydrous and acetonitrile, and centrifuged. The supernatant was isolated, rinsed with n-hexane saturated with acetonitrile, and evaporated. The residue was dissolved with 0.1 M potassium dihydrogenphosphate, and 0.1 M sodium hydrogencarbonate, and then cleaned up on a Bond Elut C-18 cartridge. The three compounds were separated on a Capcell Pak C-18 UG 120 (5 mu m, 150x4.6 mm I.D.) column and determined by UV detection at 295 nm, The mobile phase was acetonitrile-0.05 M ammoniumacetate (50:50), and the how-rate was 0.8 ml/min at 40 degrees C, The meanrecoveries (n = 4) were 89.1-95.0% with a relative standard deviation of 1.1-2.6%. The detection limits were 0.004-0.006 mu g/g in milk. The proposedmethod was used to monitor raw milk samples for the market, and applied tothe analysis of milk samples from 10 cows which had been administered withtriclabendazole to control the liver fluke. The confirmation of the triclabendazole and its metabolites in the above milk sample has been achieved byelectrospray LC-MS fur the first time. (C) 2000 Elsevier Science BN. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:12:35