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Titolo:
Determination of trenbolone and zeranol in bovine muscle and liver by liquid chromatography-electrospray mass spectrometry
Autore:
Horie, M; Nakazawa, H;
Indirizzi:
Saitama Prefectural Inst Publ Hlth, Urawa, Saitama 338, Japan Saitama Prefectural Inst Publ Hlth Urawa Saitama Japan 338 ama 338, Japan Hoshi Univ, Shinagawa Ku, Tokyo 108, Japan Hoshi Univ Tokyo Japan 108Hoshi Univ, Shinagawa Ku, Tokyo 108, Japan
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 882, anno: 2000,
pagine: 53 - 62
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANABOLIC AGENTS; TISSUES; MS;
Keywords:
food analysis; trenbolone; zeranol; growth hormones;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Horie, M Saitama Prefectural Inst Publ Hlth, 639-1 Kamiokubo, Urawa, Saitama 338, Japan Saitama Prefectural Inst Publ Hlth 639-1 Kamiokubo Urawa Saitama Japan 338
Citazione:
M. Horie e H. Nakazawa, "Determination of trenbolone and zeranol in bovine muscle and liver by liquid chromatography-electrospray mass spectrometry", J CHROMAT A, 882(1-2), 2000, pp. 53-62

Abstract

A sensitive and selective method using liquid chromatography (LC)-electrospray mass spectrometry for the determination of growth promoters, trenbolone and zeranol, in bovine muscle and liver has been developed. The LC separation was performed on a Zorbax XDB-C18 column (150X2.1 mm I.D.) using 0.005% acetic acid-acetonitrile (60:40, v/v) as the mobile phase at a flow-rate of 0.2 ml/min. The positive ionization produced typical (M+H)(+) molecular ions of or-trenbolone and beta-trenbolone. On the other hand, the negative mode produced (M-H)(-) ion of zeranol. The calibration graphs for a-trenbolone, beta-trenbolone and zeranol were rectilinear from 2.5 pg to 1.0 ng with selected ion monitoring. The drugs were extracted with 0.2% metaphosphoric acid-acetonitrile (6:4, v/v), and the extracts were cleaned up on a OASISHLB (60 mg) cartridge. The recoveries of the hormones from bovine muscle fortified at 2 ng/g were 82.3-85.1%, and detection limits were 0.5 ng/g for each drug. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:14:10