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Titolo:
The salivary adenosine deaminase from the sand fly Lutzomyia longipalpis
Autore:
Charlab, R; Rowton, ED; Ribeiro, JMC;
Indirizzi:
NIAID, Parasit Dis Lab, Sect Med Entomol, NIH, Bethesda, MD 20892 USA NIAID Bethesda MD USA 20892 Sect Med Entomol, NIH, Bethesda, MD 20892 USA Walter Reed Army Med Ctr, Walter Reed Army Inst Res, Dept Entomol, Washington, DC 20307 USA Walter Reed Army Med Ctr Washington DC USA 20307 Washington, DC 20307 USA
Titolo Testata:
EXPERIMENTAL PARASITOLOGY
fascicolo: 1, volume: 95, anno: 2000,
pagine: 45 - 53
SICI:
0014-4894(200005)95:1<45:TSADFT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFICATION; EXPRESSION; IDENTIFICATION; VASODILATORS; ARTHROPODS; RECEPTORS; MAXADILAN; BINDING; CLONING; PEPTIDE;
Keywords:
Lutzomyia longipalpis; Phlebotomine sand flies; blood-feeding insects; salivary glands; adenosine deaminase (EC 3.5.4.4); adenosine; inosine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Charlab, R NIAID, Parasit Dis Lab, Sect Med Entomol, NIH, Bldg 4,Room 126,4 Ctr Dr,MSC 0425, Bethesda, MD 20892 USA NIAID Bldg 4,Room 126,4 Ctr Dr,MSC 0425 Bethesda MD USA 20892 A
Citazione:
R. Charlab et al., "The salivary adenosine deaminase from the sand fly Lutzomyia longipalpis", EXP PARASIT, 95(1), 2000, pp. 45-53

Abstract

In the process of sequencing a subtracted cDNA library from the salivary glands of the sand fly Lutzomyia longipalpis, we identified a cDNA with similarities to gene products of the adenosine deaminase family. Prompted by this cDNA finding, we detected adenosine deaminase activity at levels of 1 U/mg protein in salivary gland homogenates. The activity was significantly reduced following a blood meal indicating its apparent secretory fate. The native enzyme has a K-m of similar to 10 mu M, an isoelectric pH between 4.5 and 5.5, and an apparent molecular weight of 52 kDa by size exclusion chromatography. The possible role of this enzyme, which converts adenosine to inosine, in the feeding physiology of L. longipalpis is discussed.

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Documento generato il 09/04/20 alle ore 00:21:46