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Titolo:
Dominant expansion of human T cells in non-obese diabetic/severe combined immunodeficiency mice implanted with human bone fragments
Autore:
Fujiki, Y; Onodera, M; Yamaguchi, T; Osawa, M; Sudo, K; Hamada, H; Ema, H; Shibuya, A; Takiguchi, M; Kubo, T; Nakauchi, H;
Indirizzi:
Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Japan Sci & Technol Corp, CREST, Tsukuba, Ibaraki, Japan Japan Sci & Technol Corp Tsukuba Ibaraki Japan , Tsukuba, Ibaraki, Japan Univ Tsukuba, Inst Clin Med, Dept Obstet & Gynecol, Tsukuba, Ibaraki 305, Japan Univ Tsukuba Tsukuba Ibaraki Japan 305 necol, Tsukuba, Ibaraki 305, Japan Kumamoto Univ, Ctr AIDS Res, Div Viral Immunol, Kumamoto, Japan Kumamoto Univ Kumamoto Japan DS Res, Div Viral Immunol, Kumamoto, Japan
Titolo Testata:
EXPERIMENTAL HEMATOLOGY
fascicolo: 7, volume: 28, anno: 2000,
pagine: 792 - 801
SICI:
0301-472X(200007)28:7<792:DEOHTC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
SCID-HU MOUSE; PERIPHERAL-BLOOD LYMPHOCYTES; TERM HUMAN HEMATOPOIESIS; HUMAN IMMUNE-SYSTEM; HIV-INFECTION; MODEL; MARROW; DIFFERENTIATION; MAINTENANCE; MUTATION;
Keywords:
bone fragment; NOD/SCID; human T lymphopoiesis; HIV infection; animal model;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Nakauchi, H Univ Tsukuba, Inst Basic Med Sci, Dept Immunol, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba 1-1-1 Tennodai Tsukuba Ibaraki Japan 3058575 apan
Citazione:
Y. Fujiki et al., "Dominant expansion of human T cells in non-obese diabetic/severe combined immunodeficiency mice implanted with human bone fragments", EXP HEMATOL, 28(7), 2000, pp. 792-801

Abstract

Objective. To establish an in vivo animal model in which human T cells develop and function normally, a step toward developing new vaccines or chemical compounds that modulate immune functions and toward understanding T-cellimmunity in humans. Materials and Methods. Human bone fragments were implanted into non-obese diabetes / severe combined immunodeficiency (NOD/SCID) mice. The presence of human blood cells in the peripheral blood of these mice was monitored periodically by immunostaining and fluorescence-activated cell sorting. Results. After implantation of bone fragments, dominant expansion of humanT lymphocytes, rather than myeloid and B cells, was observed over a 3-month period. In some cases, the proportion of human T cells rose to 40% of theperipheral blood mononuclear cells, These T cells showed CD4/CD8 ratios similar to those observed in human peripheral blood lymphocytes and had a broad repertoire of rearranged T-cell receptor genes. Craft-versus-host reaction was not noted in any organ analyzed. To assess the suitability of NOD/SCID mice implanted with human bone fragments (hu-bone-NOD/SCID mice) as an in vivo model for HIV infection, the mice were infected with a T-lymphotropic strain of HIV-1 (NL4-3) at 7 weeks posttransplant. Serum p24 gag was detected at 2 weeks after inoculation, after which total CD4-positive cell numbers declined, as seen clinically in patients infected with HIV. Conclusion. Although the precise mechanism is yet to be determined by which predominant expansion of human T cells occurs in hu-bone-NOD/SCID mice, such mice appear likely to serve as a useful and versatile model for studiesinvolving human T-cell immunity. (C) 2000 International Society for Experimental Hematology. Published by Elsevier Science Inc.

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Documento generato il 18/09/20 alle ore 11:08:16