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Titolo:
Activation of spinal cannabinoid (1) receptors inhibits C-fibre driven hyperexcitable neuronal responses and increases [S-35]GTP gamma S binding in the dorsal horn of the spinal cord of noninflamed and inflamed rats
Autore:
Drew, LJ; Harris, J; Millns, PJ; Kendall, DA; Chapman, V;
Indirizzi:
Univ Nottingham, Queens Med Ctr, Sch Med, Sch Biomed Sci, Nottingham NG7 2UH, England Univ Nottingham Nottingham England NG7 2UH , Nottingham NG7 2UH, England
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 6, volume: 12, anno: 2000,
pagine: 2079 - 2086
SICI:
0953-816X(200006)12:6<2079:AOSC(R>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYNAMIC-RANGE NEURONS; MU-OPIOID RECEPTORS; INDUCED ANTINOCICEPTION; CALCIUM CHANNELS; EVOKED ACTIVITY; N-TYPE; INFLAMMATION; BLOCKADE; AGONIST; BRAIN;
Keywords:
antinociception; cannabinoid agonist HU210; carrageenan inflammation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Chapman, V Univ Nottingham, Queens Med Ctr, Sch Med, Sch Biomed Sci, E Floor, Nottingham NG7 2UH, England Univ Nottingham E Floor Nottingham EnglandNG7 2UH UH, England
Citazione:
L.J. Drew et al., "Activation of spinal cannabinoid (1) receptors inhibits C-fibre driven hyperexcitable neuronal responses and increases [S-35]GTP gamma S binding in the dorsal horn of the spinal cord of noninflamed and inflamed rats", EUR J NEURO, 12(6), 2000, pp. 2079-2086

Abstract

The analgesic potential of cannabinoid (CB) receptor agonists is of clinical interest. Improved understanding of the mechanisms of action of cannabinoids at sites involved in the modulation of acute and sustained inflammatory nociceptive transmission, such as the spinal cord, is essential. In vivo electrophysiology was used to compare the effect of the synthetic CB agonist, HU210, on acute transcutaneous electrical-evoked responses of dorsal horn neurons of noninflamed anaesthetized rats and anaesthetized rats with a peripheral carrageenin inflammation. CB receptor G-protein coupling in lumbar spinal cord sections of noninflamed and carrageenin-inflamed rats was studied with in vitro autoradiography of guanylyl 5'-[gamma-[S-35]thio]triphosphate ([S-35]GTP gamma S) binding. Spinal HU210 significantly inhibited theC-fibre-mediated late (300-800 ms) postdischarge response of dorsal horn neurons of noninflamed and carrageenin-inflamed rats; the CB1 receptor antagonist SR141716A blocked the effect of HU210. HU210 had limited effects on A-fibre-evoked dorsal horn neuronal responses of both groups of rats. HU210 significantly increased [S-35]GTP gamma S binding in the dorsal horn of thespinal cord of both groups of rats compared with basal [S-35]GTP gamma S binding; SR141716A blocked these effects. The predominant effect of spinal HU210, via CB1 receptor activation, was on the C-fibre driven postdischarge responses, a measure of neuronal hyperexcitability following repetitive C-fibre stimulation. Sustained, but not enhanced, antinociceptive effects of HU210 following carrageenin inflammation are reported; CB receptor G-proteincoupling was not altered by inflammation. These results strengthen the body of evidence suggesting CB agonists may be an important novel analgesic approach for the treatment of sustained pain states.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 04:09:38