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Titolo:
Role of desensitization of AMPA receptors on the neuronal viability and onthe [Ca (2+)](i) changes in cultured rat hippocampal neurons
Autore:
Ambrosio, AF; Silva, AP; Malva, JO; Mesquita, JF; Carvalho, AP; Carvalho, CM;
Indirizzi:
Univ Coimbra, Dept Zool, Ctr Neurosci Coimbra, P-3004517 Coimbra, PortugalUniv Coimbra Coimbra Portugal P-3004517 bra, P-3004517 Coimbra, Portugal Univ Coimbra, Fac Med, Biochem Lab, P-3004517 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3004517 Lab, P-3004517 Coimbra, Portugal Univ Coimbra, Dept Bot, Electron Microscopy Lab, P-3004517 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3004517 Lab, P-3004517 Coimbra, Portugal
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 6, volume: 12, anno: 2000,
pagine: 2021 - 2031
SICI:
0953-816X(200006)12:6<2021:RODOAR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR PURKINJE-CELLS; GLUTAMATE RECEPTORS; MEDIATED EXCITOTOXICITY; CA2+-PERMEABLE AMPA/KAINATE; KAINATE EXCITOTOXICITY; CORTICAL-NEURONS; CA2+ ENTRY; IN-VITRO; CYCLOTHIAZIDE; NEUROTOXICITY;
Keywords:
cyclothiazide; kainate; LY 303070; neuroprotection; neurotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Carvalho, CM Univ Coimbra, Dept Zool, Ctr Neurosci Coimbra, P-3004517 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3004517 7 Coimbra, Portugal
Citazione:
A.F. Ambrosio et al., "Role of desensitization of AMPA receptors on the neuronal viability and onthe [Ca (2+)](i) changes in cultured rat hippocampal neurons", EUR J NEURO, 12(6), 2000, pp. 2021-2031

Abstract

We investigated the role of desensitization of alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate (AMPA) receptors on the neurotoxicity and on the[Ca2+](i) changes induced by kainate or by AMPA in cultured rat hippocampal neurons. The neuronal viability was evaluated either by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, or by analysisof cell morphology. Short-term exposure of the neurons to kainate or AMPA (30 min) was not toxic, but the exposure for 24 h to the excitotoxic drugs caused a concentration-dependent neurotoxic effect which was prevented by LY 303070, a noncompetitive AMPA receptor antagonist. In the presence of cyclothiazide (CTZ), kainate or AMPA was toxic (30 min exposure), or the toxiceffect was significantly enhanced (24 h exposure), but in this case LY 303070 did not completely protect the cells against kainate-induced toxicity. The alterations in the [Ca2+](i) caused by kainate or AMPA showed a great cell-to-cell variability. LY 303070 completely or partially inhibited the responses stimulated by kainate. CTZ differentially affected the responses evoked by kainate or AMPA. In the majority of hippocampal neurons, CTZ did not potentiate, or only slightly potentiated, the kainate-stimulated responses but in 11% of neurons there was a great potentiation. In AMPA-stimulated neurons, the responses were slightly or greatly potentiated in the majorityof neurons, but not in all of them. The results show that AMPA and kainatemay be toxic, depending on the time of exposure and on the blockade of thedesensitization of the AMPA receptors. Overall, our results clearly show that there exist different populations of hippocampal neurons with differentsensitivities to kainate, AMPA, CTZ and LY 303070. Moreover, the effects of CTZ on both [Ca2+](i) alterations and neurotoxicity are not fully correlated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:42:25