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Titolo:
A new class of neurotoxin from wasp venom slows inactivation of sodium current
Autore:
Sahara, Y; Gotoh, M; Konno, K; Miwa, A; Tsubokawa, H; Robinson, HPC; Kawai, N;
Indirizzi:
Jichi Med Sch, Dept Physiol, Mibu, Tochigi 3290498, Japan Jichi Med Sch Mibu Tochigi Japan 3290498 ol, Mibu, Tochigi 3290498, Japan Tokyo Med & Dent Univ, Fac Dent, Dept Physiol, Tokyo 1138549, Japan Tokyo Med & Dent Univ Tokyo Japan 1138549 Physiol, Tokyo 1138549, Japan Tokyo Med & Dent Univ, Fac Dent, Dept Endodont, Tokyo 1138549, Japan TokyoMed & Dent Univ Tokyo Japan 1138549 Endodont, Tokyo 1138549, Japan Sao Paulo State Univ, Inst Biosci Rio Claro, Rio Claro, Brazil Sao Paulo State Univ Rio Claro Brazil osci Rio Claro, Rio Claro, Brazil Tokyo Metropolitan Inst Neurosci, Dept Neurobiol, Tokyo 1838536, Japan Tokyo Metropolitan Inst Neurosci Tokyo Japan 1838536 okyo 1838536, Japan Univ Cambridge, Physiol Lab, Cambridge CB2 3EG, England Univ Cambridge Cambridge England CB2 3EG Lab, Cambridge CB2 3EG, England
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 6, volume: 12, anno: 2000,
pagine: 1961 - 1970
SICI:
0953-816X(200006)12:6<1961:ANCONF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHANNEL ALPHA-SUBUNIT; SEA-ANEMONE TOXIN; SCORPION TOXIN; NEUROMUSCULAR SYNAPSE; GLUTAMATE-RECEPTOR; CATIONIC RESIDUES; GANGLION NEURONS; ANTHOPLEURIN-B; SOLITARY WASP; HIGH-AFFINITY;
Keywords:
inactivation; lobster neuromuscular synapse; sodium channel; trigeminal ganglion; wasp toxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Kawai, N Jichi Med Sch, Dept Physiol, Mibu, Tochigi 3290498, Japan Jichi Med Sch Mibu Tochigi Japan 3290498 Tochigi 3290498, Japan
Citazione:
Y. Sahara et al., "A new class of neurotoxin from wasp venom slows inactivation of sodium current", EUR J NEURO, 12(6), 2000, pp. 1961-1970

Abstract

The effects of alpha-pompilidotoxin (alpha-PMTX), a new neurotoxin isolated from the venom of a solitary wasp, were studied on the neuromuscular synapses in lobster walking leg and the rat trigeminal ganglion (TG) neurons. Paired intracellular recordings from the presynaptic axon terminals and the innervating lobster leg muscles revealed that alpha-PMTX induced long bursts of action potentials in the presynaptic axon, which resulted in facilitated excitatory and inhibitory synaptic transmission. The action or alpha-PMTX was distinct from that of other known facilitatory presynaptic toxins, including sea anemone toxins and alpha-scorpion toxins, which modify the fastinactivation of Na+ current. We further characterized the action of alpha-PMTX on Na+ channels by whole-cell recordings from rat trigeminal neurons. We found that alpha-PMTX stowed the Na+ channels inactivation process without changing the peak current-voltage relationship or the activation time course of tetrodotoxin (TTX)-sensitive Na+ currents, and that alpha-PMTX had voltage-dependent effects on the rate of recovery from Na+ current inactivation and deactivating tail currents. The results suggest that alpha-PMTX slows or blocks conformational changes required for fast inactivation of the Na+ channels on the extracellular surface. The simple structure of alpha-PMTX, consisting of 13 amino acids, would be advantageous for understanding the functional architecture of Na+ channel protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:15:16