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Titolo:
The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family
Autore:
Tanaka, K; Okabayashi, K; Asashima, M; Perrimon, N; Kadowaki, T;
Indirizzi:
Nagoya Univ, Grad Sch Bioagr Sci, Grad Program Regulat Biol Signals, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601a Ku, Nagoya, Aichi 4648601, Japan Univ Tokyo, Dept Life Sci, Tokyo, Japan Univ Tokyo Tokyo JapanUniv Tokyo, Dept Life Sci, Tokyo, Japan Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Genet, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 d Inst, Dept Genet, Boston, MA 02115 USA
Titolo Testata:
EUROPEAN JOURNAL OF BIOCHEMISTRY
fascicolo: 13, volume: 267, anno: 2000,
pagine: 4300 - 4311
SICI:
0014-2956(200007)267:13<4300:TECPGF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-SURFACE; WINGLESS PROTEIN; DROSOPHILA; EXPRESSION; GLYCOSAMINOGLYCANS; BIOSYNTHESIS; MUTATIONS; ENDODERM; REVEALS; EMBRYOS;
Keywords:
alternative splicing; chaperone; Porcupine; Wnt family;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Kadowaki, T Nagoya Univ, Grad Sch Bioagr Sci, Grad Program Regulat Biol Signals, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 , Aichi 4648601, Japan
Citazione:
K. Tanaka et al., "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family", EUR J BIOCH, 267(13), 2000, pp. 4300-4311

Abstract

The Drosophila segment polarity gene product Porcupine (Porc) was first identified as being necessary for processing Wingless (Wg), a Drosophila Wnt (Wnt) family member. Mouse and Xenopus homologs of porc (Mporc and Xporc) were identified and found to encode endoplasmic reticulum (ER) proteins withmultiple transmembrane domains. In contrast with porc, four different types of Mporc and Xporc mRNA (A-D) are generated from a single gene by alternative splicing. Mporc mRNA is differentially expressed during embryogenesis and in various adult tissues, demonstrating that the alternative splicing is regulated to synthesize the specific types of Mporc. In transfected mammalian cells, all Mporc types affect the processing of mouse Wnt 1, 3A, 4, 6,and 7B but not 5A. Furthermore, all Mporc types are co-immunoprecipitated with various Wnt proteins. These results suggest that Mporc may function asa chaperone-like molecule for Wnt. Interestingly, all Mporc types can substitute for Porc, as they are able to rescue the phenotypes of Drosophila porc embryos. Consistent with this observation, Mporc, like Porc, modifies the processing of Wg expressed in mammalian cells. These results demonstrate that the porc gene family encodes the multitransmembrane ER proteins, whichare evolutionarily well conserved and involved in processing the Wnt family.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:06:33