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Titolo:
Enhanced stimulation of glucose uptake by insulin increases exercise-stimulated glucose uptake in skeletal muscle in humans - Studies using [O-15]O-2, [O-15]H2O, [F-18]fluoro-deoxy-glucose and positron emission tomography
Autore:
Nuutila, P; Peltoniemi, P; Oikonen, V; Larmola, K; Kemppainen, J; Takala, T; Sipila, H; Oksanen, A; Ruotsalainen, U; Bolli, GB; Yki-Jarvinen, H;
Indirizzi:
Univ Turku, Turku PET Ctr, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 rku PET Ctr, FIN-20520 Turku, Finland Univ Turku, Dept Med, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 u, Dept Med, FIN-20520 Turku, Finland Univ Turku, Dept Phys Med, FIN-20520 Turku, Finland Univ Turku Turku Finland FIN-20520 pt Phys Med, FIN-20520 Turku, Finland Minerva Fdn, Inst Med Res, Helsinki, Finland Minerva Fdn Helsinki Finland nerva Fdn, Inst Med Res, Helsinki, Finland Univ Helsinki, Dept Med, Helsinki, Finland Univ Helsinki Helsinki Finland iv Helsinki, Dept Med, Helsinki, Finland Univ Perugia, Dept Internal Med, I-06100 Perugia, Italy Univ Perugia Perugia Italy I-06100 Internal Med, I-06100 Perugia, Italy
Titolo Testata:
DIABETES
fascicolo: 7, volume: 49, anno: 2000,
pagine: 1084 - 1091
SICI:
0012-1797(200007)49:7<1084:ESOGUB>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-FLOW; TRANSPORT; PLASMA; HEART; CONTRACTIONS; SENSITIVITY; KINETICS; KINASE; HYPERGLYCEMIA; RESISTANCE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Nuutila, P Univ Turku, Turku PET Ctr, POB 52, FIN-20520 Turku, Finland Univ Turku POB 52 Turku Finland FIN-20520 20520 Turku, Finland
Citazione:
P. Nuutila et al., "Enhanced stimulation of glucose uptake by insulin increases exercise-stimulated glucose uptake in skeletal muscle in humans - Studies using [O-15]O-2, [O-15]H2O, [F-18]fluoro-deoxy-glucose and positron emission tomography", DIABETES, 49(7), 2000, pp. 1084-1091

Abstract

In vitro studies have shown that insulin and exercise stimulate glucose uptake in part via distinct mechanisms. We determined whether a high rate of insulin-stimulated glucose uptake (good insulin sensitivity) is associated with an enhanced ability of exercise to increase glucose uptake in vivo in humans. In our study, 22 normal subjects performed one-legged isometric exercise for 105 min (45-150 min) under intravenously maintained euglycemic-hyperinsulinemic conditions (0-150 min). Rates of oxygen consumption, blood flow, and glucose uptake were quantitated simultaneously in skeletal muscle of both legs using [O-15]O-2, [O-15]H2O, [F-18]fluoro-deoxy-glucose, and positron emission tomography. The one-legged exercise, performed at an intensity of 11% of maximal isometric force, was designed to induce similar increases in oxygen consumption in both groups. In the entire group, exercise increased oxygen consumption from 2.3 +/- 0.3 ml . kg(-1) muscle . min(-1) (insulin) to 34.2 +/- 3. ml . kg(-1) muscle . min(-1) (insulin and exercise) (P < 0.001) and muscle glucose uptake from 60 +/- 6 mu mol . kg(-1) muscle . min(-1) (insulin) to 220 +/- 22 mu mol . kg(-1) muscle . min(-1) (insulinand exercise) (P < 0.001). The exercise-induced increase in glucose uptakewas due to marked increases in blood flow (36 +/- 5 ml . kg(-1) muscle . min(-1) [insulin] vs. 262 +/- 20 ml . k(-1) muscle . min(-1) [insulin and exercise], P < 0.001) rather than glucose extraction, which decreased from 2.0 +/- 0.2 mmol/l (insulin) to 1.0 +/- 0.1 mmol/l (insulin and exercise) (P < 0.001). The subjects were classified according to their mean rate of whole-body insulin-stimulated glucose uptake into those with high (49 +/- 3 mu mol . kg(-1) . min(-1)) and normal (27 +/- 2 mu mol . kg(-1) . min(-1)) rates of insulin-stimulated glucose uptake. Both insulin-stimulated (2.4 +/- 1.1 vs. 2.3 +/- 1.2 ml . kg(-1) muscle . min(-1), normal vs. high insulin sensitivity) and exercise- and insulin-stimulated (33 +/- 6 vs. 34 +/- 4 ml .kg(-1) muscle . min(-1)) rates of oxygen consumption were comparable between the groups. Exercise increased glucose uptake more in the group with high insulin sensitivity (195 +/- 25 mu mol . kg(-1) . muscle. min(-1)) than in the group with normal insulin sensitivity (125 +/- 19 mu mol . kg(-1) muscle . min(-1)) (P < 0.05). Muscle blood flow was closely correlated with the rate of oxygen consumption (r = 0.91, P < 0.0001), and insulin-stimulated(30 +/- 5 vs. 35 +/- 6 ml . kg(-1) muscle . min(-1)) and exercise-induced increments (222 +/- 31 vs. 228 +/- 23 ml . kg(-1) muscle . min(-1)) in muscle blood flow were similar between the groups. Glucose extraction remained higher in the group with high insulin sensitivity (1.2 +/- 0.2 mmol/l) thanin the group with normal insulin sensitivity (0.7 +/- 0.1 mmol/l, P < 0.05). We conclude that whereas acute exercise per se increases glucose uptake via increasing glucose delivery, good insulin sensitivity modulates exercise-induced increases in glucose uptake by enhancing cellular glucose extraction.

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Documento generato il 20/01/20 alle ore 16:00:57