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Titolo:
How urine analysis reflects oxidative stress - nitrotyrosine as a potential marker
Autore:
Schwemmer, M; Fink, B; Kockerbauer, R; Bassenge, E;
Indirizzi:
Univ Freiburg, Inst Appl Physiol, D-79104 Freiburg, Germany Univ FreiburgFreiburg Germany D-79104 hysiol, D-79104 Freiburg, Germany
Titolo Testata:
CLINICA CHIMICA ACTA
fascicolo: 1-2, volume: 297, anno: 2000,
pagine: 207 - 216
SICI:
0009-8981(200007)297:1-2<207:HUAROS>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; SUPEROXIDE-DISMUTASE; TYROSINE NITRATION; VITAMIN-C; PEROXYNITRITE; 3-NITROTYROSINE; TOLERANCE; ATHEROSCLEROSIS; RADICALS; NITROSATION;
Keywords:
reactive oxygen species; reactive nitrogen species; superoxide dismutase; nitration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Schwemmer, M Univ Freiburg, Inst Appl Physiol, Hermann Herder Str 7, D-79104 Freiburg, Germany Univ Freiburg Hermann Herder Str 7 Freiburg Germany D-79104
Citazione:
M. Schwemmer et al., "How urine analysis reflects oxidative stress - nitrotyrosine as a potential marker", CLIN CHIM A, 297(1-2), 2000, pp. 207-216

Abstract

Enhanced oxidant stress involved in the pathogenesis of cardiovascular (heart failure, atherosclerosis, ischemia-reperfusion injury), neurodegenerative (M. Alzheimer), metabolic (hyper-cholesterolemia, diabetes) and inflammatory disorders is mimicked by non-intermittent therapy with nitrovasodilators. We used this latter therapy model to study urinary 3-nitrotyrosine (n-tyr) excretion as a potential biomarker that may reflect the enhanced generation of reactive oxygen species. Namely, free or protein-bound n-tyr is formed in the organism by nitration of tyrosine (residues) via peroxynitrite (reaction product of NO . and O-2.). Free n-tyr content was analyzed by gas chromatography in urine obtained from healthy human subjects under a nitrite-limited diet during a two-day non-intermittent transdermal administrationof glyceroltrinitrate (GTN; 0.4 mg/h) with or without vitamin C (Vit-C; 55mu g/kg/min) as antioxidant. Concomitant with the development of complete vascular tolerance (loss of dilator action), a progressive increase in urinary n-tyr excretion (up to 186+/-9 mu g/day) was demonstrated in volunteersgiven GTN only. In contrast, when Vit-C was added, the GTN-induced increases in urinary n-tyr content were significantly suppressed (up to 130.20+/-6.91 mu g/day), whereas Vit-C alone even decreased urinary a-tyr content (down to 34.00+/-5.66 mu g/day), which was below control values (56.0+/-3.4 mug/day). Thus, urinary n-tyr may serve as a biomarker to detect changes in oxidant stress and thereby to evaluate the efficacy of therapeutic interventions aimed at reducing oxidant stress under various pathophysiological conditions. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 09:44:24