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Titolo:
AP-1 mediated signal transduction in thrombin-induced regulation of PAL-1 expression in human mesangial cells
Autore:
Chen, XM; He, QN; Liu, WH; Xu, QH; Ye, YZ; Fu, B; Yu, LF;
Indirizzi:
Gen Hosp Chinese PLA, Dept Nephrol, Beijing 100853, Peoples R China Gen Hosp Chinese PLA Beijing Peoples R China 100853 853, Peoples R China
Titolo Testata:
CHINESE MEDICAL JOURNAL
fascicolo: 6, volume: 113, anno: 2000,
pagine: 514 - 519
SICI:
0366-6999(200006)113:6<514:AMSTIT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR INHIBITOR-1; GENE-EXPRESSION; HEPG2 CELLS; KAPPA-B; PROTEINS; KINASE; SYSTEM; PAI-1;
Keywords:
thrombin; activator protein-1; plasminogen activator inhibitor-1; mesangial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, XM Gen Hosp Chinese PLA, Dept Nephrol, 28 Fuxing Rd, Beijing 100853,Peoples R China Gen Hosp Chinese PLA 28 Fuxing Rd Beijing Peoples R China100853
Citazione:
X.M. Chen et al., "AP-1 mediated signal transduction in thrombin-induced regulation of PAL-1 expression in human mesangial cells", CHIN MED J, 113(6), 2000, pp. 514-519

Abstract

Objective To evaluate activator protein-1 (AP-1) mediated mechanisms in thrombin-induced qlasminogen activator inhibitor-1 (PAI-1) expression in cultured human glomerular mesangial cells (MCs). Methods Electrophoretic mobility shift assay (EMSA) was employed to assessAP-1 DNA-binding activity, and Western blot hybridization was used for quantification of c-fos and c-jun, two subunits of AP-1 dimers. PAI-1 activityand mRNA expression were analysed by the fibrin plate assay and Northern hybridization, respectively. Results Thrombin concentration enhanced PAI-1 activity in the supernatant and stimulated PAI-1 mRNA expression in cultured MCs. PAI-1 activity was blocked by hirudin, a specific inhibitor of thrombin. Further study demonstrated that thrombin promoted AP-1 DNA-binding activity but exerted little effect on c-fos or c-jun. Curcumin (AP-1 inhibitor), staurosporine (PKC inhibitor), and genistein (PTK inhibitor) all reduced AP-l-mediated PAI-1 mRNA expression induced by thrombin in cultured MCs. Conclusion The present study indicates that in cultured human MCs, thrombin stimulates PAI-1 expression through an AP-1 signal pathway, which may be mediated by PKC and PTK.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:39:05