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Titolo:
Inhibition of TGF-beta-induced apoptosis by ethinyl estradiol in cultured,precision cut rat liver slices and hepatocytes
Autore:
Chen, JQ; Gokhale, M; Schofield, B; Odwin, S; Yager, JD;
Indirizzi:
Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Environm Hlth Sci, Div Toxicol Sci, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 ol Sci, Baltimore, MD 21205 USA
Titolo Testata:
CARCINOGENESIS
fascicolo: 6, volume: 21, anno: 2000,
pagine: 1205 - 1211
SICI:
0143-3334(200006)21:6<1205:IOTABE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; NF-KAPPA-B; PROGRAMMED CELL-DEATH; LONG-TERM EXPOSURE; PEROXISOME PROLIFERATOR; DNA-SYNTHESIS; TUMOR PROMOTION; FEMALE RATS; EXPRESSION; HEPATOCARCINOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Yager, JD Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Environm Hlth Sci,Div Toxicol Sci, 615 N Wolfe St, Baltimore, MD 21205 USA Johns Hopkins Univ 615 N Wolfe St Baltimore MD USA 21205 205 USA
Citazione:
J.Q. Chen et al., "Inhibition of TGF-beta-induced apoptosis by ethinyl estradiol in cultured,precision cut rat liver slices and hepatocytes", CARCINOGENE, 21(6), 2000, pp. 1205-1211

Abstract

Ethinyl estradiol (EE) is a strong promoter of hepatocarcinogenesis in therat. Treatment with EE and other hepatic promoters induces transient growth stimulation followed by growth inhibition (mitosuppression) in hepatocytes, Previously, we identified several genes whose transcript levels were increased during EE-induced mitosuppression, including transforming growth factor beta (TGF-beta), which inhibits growth and induces apoptosis in hepatocytes. Various hepatic promoters, including phenobarbital and several peroxisomal proliferators, have been shown to inhibit TGF-beta-induced apoptosis in rat hepatocytes, The goal of this study was to investigate whether EE isalso an inhibitor of TGF-beta-induced apoptosis in rat hepatocytes. Several approaches to detect apoptosis were used, including the TUNEL assay, detection of high molecular weight DNA fragmentation by field inversion gel electrophoresis and determination of cytosolic cytochrome c levels by western analysis. TGF-beta-induced apoptosis in cultured, precision cut liver slices and hepatocytes of female rats. EE (less than or equal to 3 mu M) completely inhibited TGF-beta-induced apoptosis in these systems in the absence ofcytotoxicity, These findings add EE to the list of several hepatic promoters that both induce TGF-beta while simultaneously inhibiting its ability tocause apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:34:12