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Titolo:
8-Hydroxy-2 '-deoxyguanosine in cervical cells: correlation with grade of dysplasia and human papillomavirus infection
Autore:
Romano, G; Sgambato, A; Mancini, R; Capelli, G; Giovagnoli, MR; Flamini, G; Boninsegna, A; Vecchione, A; Cittadini, A;
Indirizzi:
Catholic Univ, Sch Med, Inst Gen Pathol, Giovanni XXIII Canc Res Ctr, I-00168 Rome, Italy Catholic Univ Rome Italy I-00168 XXIII Canc Res Ctr, I-00168 Rome, Italy Catholic Univ, Sch Med, Inst Hyg, I-00168 Rome, Italy Catholic Univ RomeItaly I-00168 Sch Med, Inst Hyg, I-00168 Rome, Italy Univ La Sapienza, Cytopathol Dept Expt Med 1, I-00161 Rome, Italy Univ La Sapienza Rome Italy I-00161 Dept Expt Med 1, I-00161 Rome, Italy
Titolo Testata:
CARCINOGENESIS
fascicolo: 6, volume: 21, anno: 2000,
pagine: 1143 - 1147
SICI:
0143-3334(200006)21:6<1143:8'ICCC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE DNA-DAMAGE; 8-HYDROXYGUANINE; 8-HYDROXYDEOXYGUANOSINE; CARCINOGENESIS; ANTIBODIES; CARCINOMA; ETIOLOGY; CANCER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Romano, G Catholic Univ, Sch Med, Inst Gen Pathol, Giovanni XXIII Canc ResCtr, L F Vito 1, I-00168 Rome, Italy Catholic Univ L F Vito 1 Rome Italy I-00168 I-00168 Rome, Italy
Citazione:
G. Romano et al., "8-Hydroxy-2 '-deoxyguanosine in cervical cells: correlation with grade of dysplasia and human papillomavirus infection", CARCINOGENE, 21(6), 2000, pp. 1143-1147

Abstract

In this study, the 8-hydroxy-2'-deoxyguanosine (8-OHdG) level was assessedin human cervical cells by an immunoperoxidase method and was related to the presence of human papillomavirus (HPV) infection and precancerous lesions. After optimizing the immunohistochemical method of detecting oxidative DNA damage in whole cells, we have used this technique to estimate the oxidative damage in cervical cells collected during a routine PAP test. The analysis of variance (ANOVA) of the data from human samples showed significant differences in the 8-OHdG content among normal, low-grade and high-grade squamous intraepithelial lesion (SIL, HGSIL and LGSIL, respectively; P < 0.001). In the comparison of the three groups, statistically significant differences were detected between normal SIL and HGSIL (P < 0.001) and between LGSIL and HGSIL (P = 0.003), whereas no statistically significant difference was found between normal SIL and LGSIL (P = 0.1). Grouping observations by HPV status, no significant difference was detected in 8-OHdG levels betweenHPV+ and HPV- subjects (P = 0.8). The polytomous and proportional odds models, extensions of the logistic regression analysis, showed that the effectof 8-OHdG levels in rising the risk of dysplasia was roughly constant through SIL grades. In conclusion, the immunoperoxidase method, applied to single human cervical cells, provides clear evidence that significant differences exist in 8-OHdG content between normal and dysplastic cells and that oxidative DNA damage might play an important role in cervical carcinogenesis.

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Documento generato il 05/12/20 alle ore 20:13:14