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Titolo:
Apoptosis and cytokine release induced by ionizing or ultraviolet B radiation in primary and immortalized human keratinocytes
Autore:
Petit-Frere, C; Capulas, E; Lyon, DA; Norbury, CJ; Lowe, JE; Clingen, PH; Riballo, E; Green, MHL; Arlett, CF;
Indirizzi:
Univ Brighton, Sch Pharm & Biomol Sci, Brighton BN2 4GJ, E Sussex, EnglandUniv Brighton Brighton E Sussex England BN2 4GJ N2 4GJ, E Sussex, England Univ Sussex, MRC, Cell Mutat Unit, Brighton BN1 9RR, E Sussex, England Univ Sussex Brighton E Sussex England BN1 9RR BN1 9RR, E Sussex, England Imperial Canc Res Fund, Videomicroscopy Lab, London WC2A 3PX, England Imperial Canc Res Fund London England WC2A 3PX London WC2A 3PX, England Univ Oxford, John Radcliffe Hosp, Inst Mol Med, ICRF Mol Oncol Lab, OxfordOX3 9DS, England Univ Oxford Oxford England OX3 9DS Mol Oncol Lab, OxfordOX3 9DS, England
Titolo Testata:
CARCINOGENESIS
fascicolo: 6, volume: 21, anno: 2000,
pagine: 1087 - 1095
SICI:
0143-3334(200006)21:6<1087:AACRIB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; ATAXIA-TELANGIECTASIA FIBROBLASTS; HUMAN CELLULAR RADIOSENSITIVITY; CULTURED HUMAN KERATINOCYTES; DOUBLE-STRAND BREAKS; DNA-DAMAGE TRIGGERS; GAMMA-IRRADIATION; FACTOR-ALPHA; IN-VITRO; DEREGULATED APOPTOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Green, MHL Univ Brighton, Sch Pharm & Biomol Sci, Cockcroft Bldg, BrightonBN2 4GJ, ESussex, England Univ Brighton Cockcroft Bldg Brighton E Sussex England BN2 4GJ
Citazione:
C. Petit-Frere et al., "Apoptosis and cytokine release induced by ionizing or ultraviolet B radiation in primary and immortalized human keratinocytes", CARCINOGENE, 21(6), 2000, pp. 1087-1095

Abstract

We have compared the induction of apoptosis and cytokine release by UVB and gamma-radiation in primary (untransformed) and in two immortalized human epithelial/keratinocyte cell lines, HaCaT and KB (KB is now known to be a subline of the ubiquitous keratin-forming tumour cell line HeLa and we therefore designate it HeLa-KB). In both the primary and the immortalized cell lines apoptosis and release of the inflammatory cytokine interleukin-6 are induced rapidly following UVB irradiation. In contrast, only the immortalized cells undergo apoptosis and release interleukin-6 after gamma-irradiationand here the onset of apoptosis and cytokine release are delayed. The samedistinction between primary and immortalized cells was observed when double-strand breaks were induced with the anticancer drug mitoxantrone, which stabilizes topoisomerase II-cleavable complexes. We suggest that immortalization may sensitize keratinocytes to the apoptogenic effect of ionizing radiation or mitoxantrone by deregulating normal cell cycle checkpoints. In both human keratinocytes and fibroblasts, cell killing, as assayed by loss of colony-forming ability, is not coupled to apoptosis, Immortalization increases resistance to gamma-radiation killing but sensitizes to apoptosis. In contrast, although immortalization also sensitizes to UVB-induced apoptosis,it does not affect UVB-induced cell killing. Apoptosis unambiguously indicates death at the single cell level but clonal cell survival integrates allthe cellular and genetic processes which prevent or permit a scorable clone to develop.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 14:37:37