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Titolo:
Genes upregulated during castration-induced rat prostatic apoptosis: cloning and characterization of new cDNAs
Autore:
Bruyninx, M; Ammar, H; Reiter, E; Cornet, A; Closset, J;
Indirizzi:
Univ Liege, Inst Pathol B23, Lab Endocrinol, Dept Biochem, B-4000 Liege, Belgium Univ Liege Liege Belgium B-4000 nol, Dept Biochem, B-4000 Liege, Belgium
Titolo Testata:
BJU INTERNATIONAL
fascicolo: 9, volume: 85, anno: 2000,
pagine: 1134 - 1142
SICI:
1464-4096(200006)85:9<1134:GUDCRP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; PROGRAMMED CELL-DEATH; T-LYMPHOMA CELLS; I IGF-I; VENTRAL PROSTATE; BINDING-PROTEINS; CANCER CELLS; EPITHELIAL-CELLS; MESSENGER-RNA; UP-REGULATION;
Keywords:
programmed cell death; castration; prostate; thymus; IGFBP; differential display;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Bruyninx, M INRA, Unite Biochim & Biol Mol Cereales, 2 Pl Viala, F-34060 Montpellier, France INRA 2 Pl Viala Montpellier France F-34060 ontpellier, France
Citazione:
M. Bruyninx et al., "Genes upregulated during castration-induced rat prostatic apoptosis: cloning and characterization of new cDNAs", BJU INT, 85(9), 2000, pp. 1134-1142

Abstract

Objective To isolate new cDNAs corresponding to genes whose expression is increased during castration-induced rat prostate apoptosis. Materials and methods Differential display of mRNAs from 3-day castrated and normal rat ventral prostates was used to identify differentially expressed clones. Northern blots were hybridized to confirm the positive regulation of the candidates and to follow the change in their expression in the involuting rat prostate, and in thymocytes of dexamethazone-treated rats. Results Five cDNAs were cloned: one encoding ribosomal protein L7, one coding for the insulin-like growth factor binding protein-3 (IGFBP-3), and three whose products are unknown. After castration, all five genes had expression kinetics that closely paralleled the proportion of prostatic epithelialcells undergoing apoptosis. The gene encoding L7 and two of the unknown genes were also upregulated in glucocorticoid-induced programmed death in thymocytes. In addition to the IGFBP-3 gene, those coding for proteins IGFBP-4, -5 and -6 were also overexpressed in the involuting prostate of androgen-deprived rats. Conclusion Five new genes were identified that are upregulated during castration-induced rat prostate apoptosis, three of which are potentially involved in the common intracellular pathway leading to programmed cell death.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:28:32