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Titolo:
Increased expression of insulin-like growth factor binding protein-1 during induced regression of bovine corpora lutea
Autore:
Sayre, BL; Taft, R; Inskeep, EK; Killefer, J;
Indirizzi:
W Virginia Univ, Div Anim & Vet Sci, Morgantown, WV 26506 USA W Virginia Univ Morgantown WV USA 26506 Vet Sci, Morgantown, WV 26506 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 1, volume: 63, anno: 2000,
pagine: 21 - 29
SICI:
0006-3363(200007)63:1<21:IEOIGF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
OVINE CORPUS-LUTEUM; ACUTE REGULATORY PROTEIN; POSTPARTUM BEEF-COWS; T-LYMPHOMA CELLS; I IGF-I; MESSENGER-RNA; ESTROUS-CYCLE; ENZYME-ACTIVITY; PROSTAGLANDIN-F2-ALPHA; PROGESTERONE;
Keywords:
corpus luteum; corpus luteum function;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Inskeep, EK W Virginia Univ, Div Anim & Vet Sci, POB 6108, Morgantown, WV 26506 USA W Virginia Univ POB 6108 Morgantown WV USA 26506 WV 26506 USA
Citazione:
B.L. Sayre et al., "Increased expression of insulin-like growth factor binding protein-1 during induced regression of bovine corpora lutea", BIOL REPROD, 63(1), 2000, pp. 21-29

Abstract

Three experiments were conducted to examine gene expression during inducedluteal regression in the cow; the initial purpose was the identification of potential embryotoxins. In experiment 1, changes in gene expression in the corpus luteum (CL) were identified by differential display reverse transcription-polymerase chain reaction (DD-PCR) during the first 72 h of luteal regression in cows treated with prostaglandin F-2 alpha (PGF(2 alpha)) on Days 4-7 after estrus. Expression of insulin-like growth factor-binding protein-1 (IGFBP-1) was up-regulated, with greatest expression at 24 h (P < 0.05) after treatment with PCF,, began. In experiment. 2, IGFBP-1 and its mRNAwere quantified in CL collected 24 or 48 h after treatment with PGF(2 alpha) on Day 4 or 10 after estrus. Because local mechanisms for exchange of hormones between the ovary and uterus are known in ruminants, uterine flushings were assayed for IGFBP-1 to seek evidence of local transfer of luteal IGFBP-1 to the uterus, IGFBP-1 mRNA was increased (P < 0.05) in CL 24 h aftertreatment when PGF(2 alpha) that began on Day 10, and by 48 h after treatment that began on Day 4, Concentrations of IGFBP-1 increased (P < 0.05) in a pattern similar to mRNA, by 24 h on Day 10, and by 48 h on Day 4. Concentrations of IGFBP-1 in uterine flushings did not change on either day. Concentrations of progesterone decreased (P < 0.05) by 8 h after treatment with PGF(2 alpha) that began on Day in, but not until 24 h after treatment that began on Day 4. In experiment 3, cows received either saline or PGF(2 alpha) and CL were collected 2 or 10 h after a single treatment, or 2 h after a second treatment that was given 8 h after the first. Expression of IGFBP-1 was increased by 2 h after treatment with PGF(2 alpha) on both Days 4 and 10 after estrus. In conclusion, secretion of IGFBP-1 is increased during luteolysis, and may inhibit the steroidogenic effects of insulin-like growth factor-I (IGF-I), but no evidence was found to implicate IGFBP-1 in the embryotoxic effect of regressing CL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 02:27:00