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Titolo:
Coatomer vesicles are not required for inhibition of Golgi transport by G-protein activators
Autore:
Happe, S; Cairns, M; Roth, R; Heuser, J; Weidman, P;
Indirizzi:
St Louis Univ, Sch Med, Dept Mol Biol & Biochem, St Louis, MO 63104 USA StLouis Univ St Louis MO USA 63104 iol & Biochem, St Louis, MO 63104 USA Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USAWashington Univ St Louis MO USA 63110 l & Physiol, St Louis, MO 63110 USA
Titolo Testata:
TRAFFIC
fascicolo: 4, volume: 1, anno: 2000,
pagine: 342 - 353
SICI:
1398-9219(200004)1:4<342:CVANRF>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSYLATION FACTOR; GTP-BINDING PROTEIN; HETEROTRIMERIC G-PROTEINS; TRIMERIC G-PROTEINS; BREFELDIN-A; ENDOPLASMIC-RETICULUM; ENDOSOME FUSION; MOLECULAR CHARACTERIZATION; VESICULAR TRANSPORT; SENSITIVE PROTEIN;
Keywords:
ADP-ribosylating factor; aluminum fluoride.; coatomer; Golgi; membrane fusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Weidman, P St Louis Univ, Sch Med, Dept Mol Biol & Biochem, St Louis, MO 63104 USA St Louis Univ St Louis MO USA 63104 em, St Louis, MO 63104 USA
Citazione:
S. Happe et al., "Coatomer vesicles are not required for inhibition of Golgi transport by G-protein activators", TRAFFIC, 1(4), 2000, pp. 342-353

Abstract

The G-protein activators guanosine 5'-O-(3-thiodiphosphate) (GTP gamma S) and aluminum fluoride (AIF) are thought to inhibit transport between Golgi cisternae by causing the accumulation of nonfunctional coatomer-coated transport vesicles on the Golgi. Although GTP gamma S and AIF inhibit transportin cell-free intra-Golgi transport systems, blocking coatomer vesicle formation does not. We therefore determined whether inhibition of in vitro Golgi transport by these agents requires coatomer vesicle formation. Depletion of coatomer was found to completely block coated vesicle formation on Golgicisternae without affecting inhibition of in vitro transport by either GTPgamma S or AIF. Depletion of ADP-ribosylation factor (ARF) prevented inhibition of transport by GTP gamma S. but not by Air, suggesting that the AIF-sensitive component in transport may not be a GTP-binding protein. Surprisingly, depletion of cytosolic ARF did not prevent the GTP gamma S-induced formation of Golgi-coated vesicles. whereas ARF was required for AIF-induced vesicle formation. Although ARF or coatomer depletion caused an increase inthe fenestration of cisternae, no other utrastructural changes were observed that might explain the inhibition of transport by GTP gamma S or Air. These findings suggest that ARF-GTP gamma S and AIF act by distinct and coatomer-independent mechanisms to inhibit membrane fusion in cell-free intra-Golgi transport.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:03:49