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Titolo:
Production of interleukin (IL)-6 and IL-8 by a choriocarcinoma cell line, BeWo
Autore:
Fujisawa, K; Nasu, K; Arima, K; Sugano, T; Narahara, H; Miyakawa, I;
Indirizzi:
Oita Med Univ, Dept Obstet & Gynecol, Oita 8795593, Japan Oita Med Univ Oita Japan 8795593 t Obstet & Gynecol, Oita 8795593, Japan
Titolo Testata:
PLACENTA
fascicolo: 4, volume: 21, anno: 2000,
pagine: 354 - 360
SICI:
0143-4004(200005)21:4<354:POI(AI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN CHORIONIC-GONADOTROPIN; CYTOKINE PRODUCTION; GROWTH-INHIBITION; HUMAN TROPHOBLAST; GENE-EXPRESSION; HUMAN-PLACENTA; CANCER-CELLS; RELEASE; CARCINOMAS; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Nasu, K Oita Med Univ, Dept Obstet & Gynecol, Oita 8795593, Japan Oita MedUniv Oita Japan 8795593 & Gynecol, Oita 8795593, Japan
Citazione:
K. Fujisawa et al., "Production of interleukin (IL)-6 and IL-8 by a choriocarcinoma cell line, BeWo", PLACENTA, 21(4), 2000, pp. 354-360

Abstract

To clarify the biological and pathological features of choriocarcinoma, weevaluated the in vitro production of cytokines by a choriocarcinoma cell line, BeWo. We measured the concentration of interleukin (IL)-6 and IL-8 in the culture media of BeWo cells after stimulation with various modulatory agents of cytokine expression by enzyme-linked immunosorbent assays. Northern blot analysis was used to examine the expression of IL-6 and IL-8 mRNA inthese cells. A weak expression of IL-6 and IL-8 was detected in unstimulated BeWo cells by both methods. IL-6 transcription and secretion were dose-dependently enhanced by stimulation with IL-1 beta, tumour necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta 1 and 12-O-tetradecanoyl phorbol-13-acetate (TPA). Forskolin, lipopolysaccharide and interferon-gamma had no effect on these cytokines production. The TNF-alpha-induced secretion of IL-6 was inhibited by dexamethasone. The TPA-induced production of IL-6 was inhibited by 1-(5-isoquinolinylsulfonyl)-2-methylpipe and sphyngosine, suggesting the involvement of a protein kinase C-dependent pathway. Levels of IL-8 mRNA and protein were also dose-dependently increased by stimulation with IL-1 beta, TNF-alpha and TPA. In contrast to IL-6, the expression of IL-8 was not affected by TGF-beta 1. It is suggested that, in additionto the production of steroidal and non-steroidal hormones, these cytokinesmay serve as part of a cytokine network that modulates the proliferation and angiogenesis of choriocarcinomas. (C) 2000 Harcourt Publishers Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 08:05:55