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Titolo:
Glutamine transport in C6 glioma cells
Autore:
Dolinska, M; Dybel, A; Albrecht, J;
Indirizzi:
Polish Acad Sci, Med Res Ctr, Dept Neurotoxicol, PL-02106 Warsaw, Poland Polish Acad Sci Warsaw Poland PL-02106 otoxicol, PL-02106 Warsaw, Poland
Titolo Testata:
NEUROCHEMISTRY INTERNATIONAL
fascicolo: 2-3, volume: 37, anno: 2000,
pagine: 139 - 146
SICI:
0197-0186(200008/09)37:2-3<139:GTICGC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-TRANSPORT; GLIAL-CELLS; SKELETAL-MUSCLE; RAT; EXPRESSION; SYSTEM; ASTROCYTES; SYNAPTOSOMES; METABOLISM; SURFACE;
Keywords:
glutamine transport; C6 cells; system ASC; system N; system L;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Albrecht, J Polish Acad Sci, Med Res Ctr, Dept Neurotoxicol, Pawinskiego 5, PL-02106 Warsaw, Poland Polish Acad Sci Pawinskiego 5 Warsaw Poland PL-02106 , Poland
Citazione:
M. Dolinska et al., "Glutamine transport in C6 glioma cells", NEUROCHEM I, 37(2-3), 2000, pp. 139-146

Abstract

Glutamine transport across the cell membranes of a variety of mammalian tissues is mediated by at least four transport systems: a sodium-independent system L, and sodium-dependent systems A, ASC and N, the latter occurring in different tissue-specific variants. In this study we assessed the contribution of these systems to the uptake of [H-3]glutamine in C6 rat glioma cells. The sodium-dependent uptake, which accounted for more than 80% of the total uptake, was not inhibited by 2-methylaminoisobutyric acid (MeAIB), indicating that system A was inactive, possibly being depressed by glutamine present in the culture medium. About 80% of the sodium-dependent uptake was mediated by system ASC, which differed from system ASC common to other CNS-and non-CNS tissues by its pH-dependence and partial lithium tolerance. The residual 20% of sodium-dependent uptake appeared to be mediated by systemN, which was identified as a component resistant to inhibition by MeAIB + threonine. The system N in CS cells appeared to be neither fully compatiblewith the neuronal system Nb, nor with the N system described in astrocytes: it differed from the former in being strongly inhibited by histidine and showing fair tolerance for lithium, and from the latter in its pH-insensitivity and strong inhibition by glutamate. The sodium-independent glutamine uptake differed from the astrocytic or neuronal uptake in its relatively weak inhibition by system L substrates and a strong inhibition by system ASC substrates, indicating a possible contribution of a variant of the ASC system. (C) 2000 Elsevier Science Ltd. All rights reserved.

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Documento generato il 28/01/21 alle ore 07:45:12